Vesicular acetylcholine transporter knock-down mice are more susceptible to pilocarpine induced status epilepticus

Vesicular acetylcholine transporter knock-down mice are more susceptible to pilocarpine induced status epilepticus

Autor Guidine, Patricia A. M. Google Scholar
Rezende, Gustavo H. S. Google Scholar
Queiroz, Claudio M. Autor UNIFESP Google Scholar
Mello, Luiz Eugenio Autor UNIFESP Google Scholar
Prado, Vania F. Google Scholar
Prado, Marco A. M. Google Scholar
Pereira, Grace S. Google Scholar
Moraes, Marcio F. D. Google Scholar
Instituição Universidade Federal de Minas Gerais (UFMG)
Universidade Federal de São Paulo (UNIFESP)
Resumo The pilocarpine (PILO) animal model of Temporal Lobe Epilepsy (TLE) portrays the most common changes in hippocampal circuitry found in human TLE. the acute cholinergic insult induces status epilepticus (SE), which triggers an overwhelming set of plastic events that result on late spontaneous recurrent limbic seizures. It has been suggested that the cholinergic system plays an important role in the synchronization required for ictogenesis. We took advantage of a knock-down animal model for the vesicular acetylcholine transporter (VAChT KD) to investigate seizure genesis in a model of cholinergic dysfunction. We induced SE in VAChT KD and wild-type (WT) mice by a single intraperitoneal injection of PILO in order to evaluate susceptibility to seizures. Video-EEG recordings evaluated epileptiform activity and ictal behavior onset. the hypothesis tested is that innate cholinergic hypofunction could result in increased susceptibility to PILO. VAChT KDHOM mice showed shorter latency for the first epileptiform discharge and for the first seizure episode, when compared to other groups. the duration of these seizure episodes, however, were not statistically different among experimental groups. On the other hand, VAChT KDHOM had the shortest latency to isoelectric EEG, when compared to WT and KDHET. Our results indicate that a reduction of brain VAChT protein to the levels found in VAChT KDHOM mice alters the epileptic response to PILO. Thus, fine-tuning modulation of cholinergic tone can affect the susceptibility of epileptic responses to pilocarpine. (c) 2008 Elsevier Ireland Ltd. All rights reserved.
Assunto vesicular acetylcholine transporter
Idioma Inglês
Data 2008-05-09
Publicado em Neuroscience Letters. Clare: Elsevier B.V., v. 436, n. 2, p. 201-204, 2008.
ISSN 0304-3940 (Sherpa/Romeo, fator de impacto)
Editor Elsevier B.V.
Extensão 201-204
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000256200800026

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