Vesicular acetylcholine transporter knock-down mice are more susceptible to pilocarpine induced status epilepticus

Vesicular acetylcholine transporter knock-down mice are more susceptible to pilocarpine induced status epilepticus

Author Guidine, Patricia A. M. Google Scholar
Rezende, Gustavo H. S. Google Scholar
Queiroz, Claudio M. Autor UNIFESP Google Scholar
Mello, Luiz Eugenio Autor UNIFESP Google Scholar
Prado, Vania F. Google Scholar
Prado, Marco A. M. Google Scholar
Pereira, Grace S. Google Scholar
Moraes, Marcio F. D. Google Scholar
Institution Universidade Federal de Minas Gerais (UFMG)
Universidade Federal de São Paulo (UNIFESP)
Abstract The pilocarpine (PILO) animal model of Temporal Lobe Epilepsy (TLE) portrays the most common changes in hippocampal circuitry found in human TLE. the acute cholinergic insult induces status epilepticus (SE), which triggers an overwhelming set of plastic events that result on late spontaneous recurrent limbic seizures. It has been suggested that the cholinergic system plays an important role in the synchronization required for ictogenesis. We took advantage of a knock-down animal model for the vesicular acetylcholine transporter (VAChT KD) to investigate seizure genesis in a model of cholinergic dysfunction. We induced SE in VAChT KD and wild-type (WT) mice by a single intraperitoneal injection of PILO in order to evaluate susceptibility to seizures. Video-EEG recordings evaluated epileptiform activity and ictal behavior onset. the hypothesis tested is that innate cholinergic hypofunction could result in increased susceptibility to PILO. VAChT KDHOM mice showed shorter latency for the first epileptiform discharge and for the first seizure episode, when compared to other groups. the duration of these seizure episodes, however, were not statistically different among experimental groups. On the other hand, VAChT KDHOM had the shortest latency to isoelectric EEG, when compared to WT and KDHET. Our results indicate that a reduction of brain VAChT protein to the levels found in VAChT KDHOM mice alters the epileptic response to PILO. Thus, fine-tuning modulation of cholinergic tone can affect the susceptibility of epileptic responses to pilocarpine. (c) 2008 Elsevier Ireland Ltd. All rights reserved.
Keywords vesicular acetylcholine transporter
Language English
Date 2008-05-09
Published in Neuroscience Letters. Clare: Elsevier B.V., v. 436, n. 2, p. 201-204, 2008.
ISSN 0304-3940 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 201-204
Access rights Closed access
Type Article
Web of Science ID WOS:000256200800026

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