Antigenotoxicity and antimutagenicity of lycopene in HepG2 cell line evaluated by the comet assay and micronucleus test

Antigenotoxicity and antimutagenicity of lycopene in HepG2 cell line evaluated by the comet assay and micronucleus test

Author Scolastici, C. Google Scholar
Lima, R. O. Alves de Google Scholar
Barbisan, L. F. Google Scholar
Ferreira, A. L. A. Google Scholar
Ribeiro, D. A. Autor UNIFESP Google Scholar
Salvadori, D. M. F. Google Scholar
Institution Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Abstract Epidemiological studies have provided evidence that high consumption of tomatoes effectively reduces the risk of reactive oxygen species (ROS)-mediated diseases such as cancer. Tomatoes are rich sources of lycopene, a potent singlet oxygen-quenching carotenoid. in addition to its antioxidant properties, lycopene shows an array of biological effects including antimutagenic and anticarcinogenic activities. in the present study, the chemopreventive action of lycopene was examined on DNA damage and clastogenic or aneugenic effects of H2O2 and n-nitrosodiethylamine (DEN) in the metabolically competent human hepatoma cell line (HepG2 cells). Lycopene at concentrations of 10. 25, and 50 mu M, was tested under three protocols: before, simultaneously, and after treatment with the mutagen, using the comet and micronucleus assays. Lycopene significantly reduced the genotoxicity and mutagenicity of H2O2 in all of the conditions tested. for DEN, significant reductions of primary DNA damage (comet assay) were detected when the carotenoid (all of the doses) was added in the cell culture medium before or simultaneously with the mutagen. in the micronucleus test, the protective effect of lycopene was observed only when added prior to DEN treatment. in conclusion, our results suggest that lycopene is a suitable agent for preventing chemically-induced DNA and chromosome damage. (C) 2007 Elsevier B.V. All rights reserved.
Keywords lycopene
DNA damage
HepG2 cells
Language English
Date 2008-03-01
Published in Toxicology in Vitro. Oxford: Pergamon-Elsevier B.V., v. 22, n. 2, p. 510-514, 2008.
ISSN 0887-2333 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 510-514
Access rights Closed access
Type Article
Web of Science ID WOS:000254694500026

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