Subretinal injection of preservative-free triamcinolone acetonide and supernatant vehicle in rabbits: an electron microscopy study

Subretinal injection of preservative-free triamcinolone acetonide and supernatant vehicle in rabbits: an electron microscopy study

Author Maia, Mauricio Autor UNIFESP Google Scholar
Penha, Fernando Marcondes Autor UNIFESP Google Scholar
Farah, Michel Eid Autor UNIFESP Google Scholar
Dib, Eduardo Autor UNIFESP Google Scholar
Principe, Andre Autor UNIFESP Google Scholar
Lima Filho, Acacio A. S. Autor UNIFESP Google Scholar
Magalhaes, Octaviano Autor UNIFESP Google Scholar
Freymueller, Edna Autor UNIFESP Google Scholar
Rodrigues, Eduardo B. Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract Background To evaluate the effect of injections of benzyl alcohol (BA)-free triamcinolone acetonide (TA) solution (MTA-PF) and the supernatant vehicle of TA (STA) containing BA into the subretinal space of rabbit eyes.Methods Sixteen rabbits underwent vitrectomy and subretinal injection of 0.02 ml of either 40 mg/ml MTA-PF, 40 mg/ml STA, or balanced salt solution (BSS). the animals were examined 6, 12, and 24 hours and 14 days after the procedure by fundus examination and fluorescein angiography (FA), as well as histological studies by light and transmission electron microscopy. the histological injury was classified in four stages: (1) stage 1, photoreceptor outer segment injury, (2) stage 2, stage 1 + photoreceptor inner segment injury, (3) stage 3, stage 2 + outer nuclear layer damage, and (4) stage 4, stage 3 + retinal pigment epithelium (RPE) damage.Results FA showed no window defects in areas where MTA-PF, STA, or BSS have been injected. Histological examination revealed that subretinal BSS-injection resulted in stage 1 damage during entire follow-up. Subretinal injection of MTA-PF resulted in damage stage 2 at 24 h and 14 days after surgery. However, at the STA position, stage 3 damage was noted 24 h and 14 days postoperatively. No RPE or choroidal damage was observed.Conclusions the histological lesions induced by subretinal STA were more relevant than the damage induced by MTA-PF. the vehicle BA may be involved in these abnormalities. the data indicate that care must be taken when using TA during internal limiting membrane peeling in macular hole surgery, due to the possibility of unintentional subretinal migration and for retinal pharmacotherapy.
Keywords triamcinolone acetonide
retina pharmacotherapy
electron microscopy
benzyl alcohol
Language English
Date 2008-03-01
Published in Graefes Archive for Clinical and Experimental Ophthalmology. New York: Springer, v. 246, n. 3, p. 379-388, 2008.
ISSN 0721-832X (Sherpa/Romeo, impact factor)
Publisher Springer
Extent 379-388
Access rights Closed access
Type Article
Web of Science ID WOS:000252992000009

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