Quantitative determination of nadolol in tablets by high-performance liquid chromatography and UV-derivative spectrophotometry

Quantitative determination of nadolol in tablets by high-performance liquid chromatography and UV-derivative spectrophotometry

Author Tsubone, Camila Autor UNIFESP Google Scholar
Garcia, Pedro Lopez Autor UNIFESP Google Scholar
Gomes, Fabio Pereira Autor UNIFESP Google Scholar
Maria Kedor-Hackmann, Erika Rosa Autor UNIFESP Google Scholar
Rocha Miritello Santoro, Maria Ines Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract High-performance liquid chromatographic (HPLC) and UV derivative spectrophotometric (UVDS) methods were developed and validated for the quantitative determination of nadolol in tablets. the HPLC method was performed on a C18 column with fluorescence detection. the excitation and emission wavelengths were 230 and 300nm, respectively. A mobile phase composed by acetonitrile-water containing 0.1% triethylamine (15:85v/v) and pH adjusted to 4.6 with formic acid was used. the UVDS method was performed taken a signal at 279.5nm. the correlation coefficient (r) obtained for both methods was 0.9999. the proposed methods are simple, precise, accurate, and can be used in routine analysis.
Keywords nadolol tablets
HPLC
UV derivative spectrophotometry
Language English
Date 2008-01-01
Published in Analytical Letters. Philadelphia: Taylor & Francis Inc, v. 41, n. 3, p. 424-436, 2008.
ISSN 0003-2719 (Sherpa/Romeo, impact factor)
Publisher Taylor & Francis Inc
Extent 424-436
Origin http://dx.doi.org/10.1080/00032710701689057
Access rights Closed access
Type Article
Web of Science ID WOS:000253084900006
URI http://repositorio.unifesp.br/handle/11600/30307

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