Diabetes induces changes of catecholamines in primary mesangial cells

Diabetes induces changes of catecholamines in primary mesangial cells

Author Di Marco, Giovana Seno Google Scholar
Colucci, Juliana Almada Autor UNIFESP Google Scholar
Fernandes, Fernanda Barrinha Autor UNIFESP Google Scholar
Vio, Carlos Pablo Google Scholar
Schor, Nestor Autor UNIFESP Google Scholar
Casarini, Dulce Elena Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Univ Clin Muenster
Pontificia Univ Catolica Chile
Abstract Diabetes mellitus is a frequent cause of kidney function damage with diabetic nephropathy being predominantly related to glomerular dysfunction. Diabetes is capable of interfering with distinct hormonal systems, as well as catecholamine metabolism. Since mesangial cells, the major constituent of renal glomerulus, constitute a potential site for catecholamine production, the present study was carried out to investigate alterations in catecholamine metabolism in cultured mesangial cells from the nonobese diabetic mouse, a well-established model for type I diabetes. We evaluated mesangial cells from normoglycemic and hyperglycemic nonobese diabetic mice, as well as cells from normoglycemic Swiss tnice as control. Mesangial cells from normoglycemic mice presented similar profiles concerning all determinations. However, cells isolated from hyperglycemic animals presented increased dopamine and norepinephrine production/secretion. Among the studied mechanisms, we observed an upregulation of tyrosine hydroxylase expression accompanied by increased tetrahydrobiopterin consumption, the tyrosine hydroxylase enzymatic cofactor. However, this increase in synthetic pathways was followed by decreased monoamine oxidase activity, which corresponds to the major metabolic pathway of catecholamines in mesangial cells. in addition, Whole kidney homogenates from diabetic animals also presented increased dopamine and norepinephrine levels when compared to normoglycemic animals. Thus, our results suggest that diabetes alters catecholamine production by interfering with both synthesizing and degrading enzymes, suggesting a possible role of catecholamine in the pathogenesis of acute and chronic renal complications of diabetes mellitus. (C) 2007 Elsevier B.V. All rights reserved.
Keywords catecholamine
mesangial cell
nonobese diabetic mice
Language English
Date 2008-01-01
Published in International Journal of Biochemistry & Cell Biology. Oxford: Pergamon-Elsevier B.V., v. 40, n. 4, p. 747-754, 2008.
ISSN 1357-2725 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 747-754
Origin http://dx.doi.org/10.1016/j.biocel.2007.10.016
Access rights Closed access
Type Article
Web of Science ID WOS:000254602600018
URI http://repositorio.unifesp.br/handle/11600/30272

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