17 beta-estradiol induces the translocation of the estrogen receptors ESR1 and ESR2 to the cell membrane, MAPK3/1 phosphorylation and proliferation of cultured immature rat Sertoli cells

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dc.contributor.author Lucas, Thais F. G. [UNIFESP]
dc.contributor.author Siu, Erica R. [UNIFESP]
dc.contributor.author Esteves, Carlos A. [UNIFESP]
dc.contributor.author Monteiro, Hugo P. [UNIFESP]
dc.contributor.author Oliveira, Cleida A.
dc.contributor.author Porto, Catarina S. [UNIFESP]
dc.contributor.author Lazari, Maria Fatima M. [UNIFESP]
dc.date.accessioned 2016-01-24T13:49:18Z
dc.date.available 2016-01-24T13:49:18Z
dc.date.issued 2008-01-01
dc.identifier http://dx.doi.org/10.1095/biolreprod.107.063909
dc.identifier.citation Biology of Reproduction. Madison: Soc Study Reproduction, v. 78, n. 1, p. 101-114, 2008.
dc.identifier.issn 0006-3363
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/30227
dc.description.abstract The aim of the present study was to determine the mechanisms involved in estrogen actions in cultured rat Sertoli cells. RT-PCR detected transcripts for the estrogen receptors ESR1 and ESR2 in cultured immature Sertoli cells and in the testis of 15-, 28-, and 120-day-old rats. the expression of ESR1 and ESR2 was confirmed in Sertoli cells by immunofluorescence and Western blot. Immunohistochemistry with cryosections of testes from immature and adult rats revealed that ESR1 is present in Sertoli, Leydig, and some peritubular myoid cells, and ESR2 is present in multiple cell types, including germ cells. Treatment of Sertoli cells with 17beta-estradiol (E-2) induced a translocation of ESR1 and ESR2 to the plasma membrane and a, concomitant phosphorylation of MAPK3/1. Both effects reached a maximum after 10 min and were blocked by PP2, an inhibitor of the SRC family of protein tyrosine kinases, and by the antiestrogen ICI 182,780 (10). MAPK3/1 phosphorylation was also decreased in the presence of AG 1478, an inhibitor of the epidermal growth factor receptor (EGFR) kinase, and in the presence of MAP2K1/2 inhibitor UO126. Treatment with 2 for 24 h increased the incorporation of [methyl-H-3]thymidine, which was blocked by ICI. These results indicate that E2 activates an SRC-mediated translocation of estrogen receptors to the plasma membrane, which results in the activation of EGFR and the mitogen-activated protein kinase signaling pathway. in addition, activation of ESR1 and/or ESR2 by E 2 is involved in proliferation of immature Sertoli cells. the estrogen actions in Sertoli cells might be a key step mediating cellular events important for spermatogenesis and fertility. en
dc.format.extent 101-114
dc.language.iso eng
dc.publisher Soc Study Reproduction
dc.relation.ispartof Biology of Reproduction
dc.rights Acesso aberto
dc.subject estradiol en
dc.subject estradiol receptor en
dc.subject kinases en
dc.subject mechanisms of hormone action en
dc.subject Sertoli cells en
dc.title 17 beta-estradiol induces the translocation of the estrogen receptors ESR1 and ESR2 to the cell membrane, MAPK3/1 phosphorylation and proliferation of cultured immature rat Sertoli cells en
dc.type Artigo
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.contributor.institution Universidade Federal de Minas Gerais (UFMG)
dc.description.affiliation Universidade Federal de São Paulo, Escola Paulista Med, Dept Pharmacol, Sect Expt Endocrinol,INFAR, BR-04044020 São Paulo, Brazil
dc.description.affiliation Universidade Federal de São Paulo, Escola Paulista Med, Dept Biochem, BR-04044020 São Paulo, Brazil
dc.description.affiliation Univ Fed Minas Gerais, Dept Morphol, BR-31270901 Belo Horizonte, MG, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Escola Paulista Med, Dept Pharmacol, Sect Expt Endocrinol,INFAR, BR-04044020 São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Escola Paulista Med, Dept Biochem, BR-04044020 São Paulo, Brazil
dc.identifier.doi 10.1095/biolreprod.107.063909
dc.description.source Web of Science
dc.identifier.wos WOS:000251916000012



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