A surface 75-kDa protein with acid phosphatase activity recognized by monoclonal antibodies that inhibit Paracoccidioides brasiliensis growth

A surface 75-kDa protein with acid phosphatase activity recognized by monoclonal antibodies that inhibit Paracoccidioides brasiliensis growth

Author Xander, Patricia Autor UNIFESP Google Scholar
Vigna, Ana Flavia Google Scholar
Feitosa, Luciano dos Santos Google Scholar
Pugliese, Livia Google Scholar
Bailao, Alexandre Melo Google Scholar
Soares, Celia Maria de Almeida Google Scholar
Mortara, Renato Arruda Google Scholar
Mariano, Mario Google Scholar
Lopes, Jose Daniel Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Universidade Federal de Goiás (UFG)
Abstract Paracoccidioides brasiliensis is a thermo-dimorphic fungus responsible for paracoccidioidomycosis (PCM), a systemic granulomatous mycosis prevalent in Latin America. the fungus releases many antigens which may be transiently bound to its cell surface. Some of them may show enzymatic functions essential for maintaining many cell processes and survival of the microorganism at different conditions. in this study, we report the characterization of a secreted 75 kDa protein from P. brasiliensis with phosphatase activity. Biologic function of the molecule was demonstrated using two specific mAbs produced and characterized as IgM and IgG isotypes. Confocal microscopy and flow cytometry analysis demonstrated that both mAbs recognized the protein on the fungus surface, mainly in its budding sites. in vitro experiments showed that fungal growth was inhibited by blocking the protein with mAbs. in addition, opsonized yeast cells with both mAbs facilitated phagocytosis by murine peritoneal macrophages. Passive immunization using mAbs before P. brasiliensis mice infection reduced colony-forming units (CFU) in the lungs as compared with controls. Histopathology showed smaller inflammation, absence of yeast cells and no granuloma formation. (c) 2007 Elsevier Masson SAS. All rights reserved.
Keywords P. brasiliensis
monoclonal antibodies
acid phosphatase
Language English
Date 2007-10-01
Published in Microbes and Infection. Amsterdam: Elsevier B.V., v. 9, n. 12-13, p. 1484-1492, 2007.
ISSN 1286-4579 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 1484-1492
Origin http://dx.doi.org/10.1016/j.micinf.2007.08.001
Access rights Closed access
Type Article
Web of Science ID WOS:000251479900013
URI http://repositorio.unifesp.br/handle/11600/30078

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