DNA damage and nitric oxide synthesis in experimentally infected balb/c mice with Trypanosoma cruzi

DNA damage and nitric oxide synthesis in experimentally infected balb/c mice with Trypanosoma cruzi

Author Ribeiro, Daniel A. Google Scholar
Calvi, Sueli A. Google Scholar
Picka, Mariele M. Google Scholar
Persi, Eliana Google Scholar
Carvalho, Thais B. de Google Scholar
Caetano, Priscila K. Google Scholar
Nagoshi, Lidiana R. Google Scholar
Lima, Carlos R. G. Google Scholar
Machado, Jussara M. Google Scholar
Salvadori, Daisy M. F. Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Univ Nacl Estadual São Paulo
Abstract This study aimed to evaluate whether experimental Chagas disease in acute phase under benznidazole therapy can cause DNA damage in peripheral blood, liver, heart, and spleen cells or induce nitric oxide synthesis in spleen cells. Twenty Balb/c mice were distributed into four groups: control (non-infected animals); Trypanosoma cruzi infected; T cruzi infected and submitted to benznidazole therapy; and only treated with benznidazole. the results obtained with the single cell gel (comet) assay showed that T cruzi was able induce DNA damage in heart cells of both benznidazole treated or untreated infected mice. Similarly, T cruzi infected animals showed an increase of DNA lesions in spleen cells. Regarding nitric oxide synthesis, statistically significant differences (p < 0.05) were observed in all experimental groups compared to negative control, the strongest effect observed in the T cruzi infected group. Taken together, these results indicate that T cruzi may increase the level of DNA damage in mice heart and spleen cells. Probably, nitric oxide plays an important role in DNA damaging whereas benznidazole was able to minimize induced T. cruzi genotoxic effects in spleen cells. (c) 2006 Elsevier Inc. All rights reserved.
Keywords Trypanosoma cruzi
Chagas disease
single cell gel (comet) assay
nitric oxide
Language English
Date 2007-07-01
Published in Experimental Parasitology. San Diego: Academic Press Inc Elsevier Science, v. 116, n. 3, p. 296-301, 2007.
ISSN 0014-4894 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 296-301
Origin http://dx.doi.org/10.1016/j.exppara.2006.12.007
Access rights Closed access
Type Article
Web of Science ID WOS:000247662500014
URI http://repositorio.unifesp.br/handle/11600/29856

Show full item record




File

File Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Search


Browse

Statistics

My Account