Distribution of Trypanosoma cruzi stage-specific epitopes in cardiac muscle of Calomys callosus, BALB/c mice, and cultured cells infected with different infective forms

Distribution of Trypanosoma cruzi stage-specific epitopes in cardiac muscle of Calomys callosus, BALB/c mice, and cultured cells infected with different infective forms

Author Taniwaki, Noerni N. Google Scholar
Silva, Claudio Vieira da Google Scholar
Silva, Solange da Google Scholar
Mortara, Renato A. Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Secao Microscopia Eletron Inst Adolfo Lutz
Abstract To examine whether distinct parasite infective forms or the mammalian host could affect the distribution of Trypanosoma cruzi stage-specific epitopes defined by monoclonal antibodies (Mabs) raised against mammalian-stage parasite forms, immunofluorescence studies followed the intracellular life cycle of the parasite in the cardiac muscle of Calomys callosus and BALB/c mice in the acute phase of the disease and in LLC-MK(2) Cultured cells. Animals and cells were infected either with tissue-culture derived trypomastigotes (TCT) or bloodstream trypornastigotes (BT) from the Y strain of T cruzi. Samples were examined under confocal fluorescence microscopy after labeling with Mabs 2C2, 1D9, 2B7, 3G8, 3B9, and 4B9 that react with carbohydrate epitopes on Ssp-4, a major amastigote surface glycoprotein; Mab 4B5 that identifies a noncarbohydrate epitope on all intracellular parasites stages, and Mab 3B2 that also recognizes a noncarbohydrate epitope expressed only in flagellated forms. Samples were double labeled with DAPI to visualize parasites' kinetoplasts and nuclei. Most of the Mabs used in this work displayed a surface labeling pattern on amastigotes present in Calomys and mice hearts, and in LLC-MK2 cultured cells infected with BT or TCT. Mab 2B7, however, displayed a marked polymorphic distribution in antigen expression between both mammalian hosts, independent on the infective form. Beyond the polymorphic distribution of amastigote surface epitopes, Calomys, and mice heart sections presented several inflammatory cells around amastigotes and trypornastigotes nests. (c) 2007 Elsevier B.V. All rights reserved.
Keywords trypanosoma cruzi
amastigote
arypomastigote
monoclonal antibody
epitope
Confocal microscopy
Language English
Date 2007-07-01
Published in Acta Tropica. Amsterdam: Elsevier B.V., v. 103, n. 1, p. 14-25, 2007.
ISSN 0001-706X (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 14-25
Origin http://dx.doi.org/10.1016/j.actatropica.2007.05.007
Access rights Closed access
Type Article
Web of Science ID WOS:000248929700002
URI http://repositorio.unifesp.br/handle/11600/29848

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