Pulsed-field gel electrophoresis in the identification of the origin of bacterial keratitis caused by Pseudomonas aeruginosa

Pulsed-field gel electrophoresis in the identification of the origin of bacterial keratitis caused by Pseudomonas aeruginosa

Author Melo, Gustavo Barreto de Google Scholar
Aggio, Fabio Bom Google Scholar
Hofling-Lima, Ana Luisa Google Scholar
d'Azevedo, Pedro Alves Autor UNIFESP Google Scholar
Campos Pignatari, Antonio Carlos Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract Purpose To report the use of pulsed-field gel electrophoresis (PFGE) in the investigation of the origin of infectious keratitis.Methods A 19-year-old girl presented with infectious keratitis in the left eye. She was a soft contact lens wearer, and was noncompliant with the proper cleaning methods proposed by the manufacturer. Microbiological assessment was performed by means of culture and PFGE.Results the patient was treated successfully with topical cefalotin and gentamicin drops. Cultures were positive for Pseudomonas aeruginosa in the cornea and conjunctiva, and in the contact lens, its case and the cleaning solution. PFGE showed identical patterns of banding in each.Conclusion in this case of bacterial keratitis, PFGE proved to be very useful in identifying how the contamination occurred.
Keywords pulsed-field gel electrophoresis
bacterial keratitis
Pseudomonas aeruginosa
Language English
Date 2007-07-01
Published in Graefes Archive for Clinical and Experimental Ophthalmology. New York: Springer, v. 245, n. 7, p. 1053-1054, 2007.
ISSN 0721-832X (Sherpa/Romeo, impact factor)
Publisher Springer
Extent 1053-1054
Origin http://dx.doi.org/10.1007/s00417-006-0503-3
Access rights Closed access
Type Article
Web of Science ID WOS:000247503800021
URI http://repositorio.unifesp.br/handle/11600/29845

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