Adjuvant requirement for successful immunization with recombinant derivatives of Plasmodium vivax merozoite surface protein-1 delivered via the intranasal route

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dc.contributor.author Bargieri, Daniel Y. [UNIFESP]
dc.contributor.author Rosa, Daniela S. [UNIFESP]
dc.contributor.author Simoes Lasaro, Melissa Ang
dc.contributor.author Ferreira, Luis Carlos S.
dc.contributor.author Soares, Irene S.
dc.contributor.author Rodrigues, Mauricio M. [UNIFESP]
dc.date.accessioned 2016-01-24T13:48:45Z
dc.date.available 2016-01-24T13:48:45Z
dc.date.issued 2007-06-01
dc.identifier http://dx.doi.org/10.1590/S0074-02762007005000039
dc.identifier.citation Memorias Do Instituto Oswaldo Cruz. Rio de Janeiro, Rj: Fundaco Oswaldo Cruz, v. 102, n. 3, p. 313-317, 2007.
dc.identifier.issn 0074-0276
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/29778
dc.description.abstract Recently, we generated two bacterial recombinant proteins expressing 89 amino acids of the C-terminal domain of the Plasmodium vivax merozoite surface protein-1 and the hexa-histidine tag (His6MSP1(19)). One of these recombinant proteins contained also the amino acid sequence of the universal pan allelic T-cell epitope (His(6)MSP1(19)-PADRE). in the present study, we evaluated the immunogenic properties of these antigens when administered via the intra-nasal route in the presence of distinct adjuvant formulations. We found that C57BL/6 mice immunized with either recombinant proteins in the presence of the adjuvants cholera toxin (CT) or the Escherichia coli heat labile toxin ( LT) developed high and long lasting titers of specific serum antibodies. the induced immune responses reached maximum levels after three immunizing doses with a prevailing IgG1 subclass response. in contrast, mice immunized by intranasal route with His(6)MSP1(19)-PADRE in the presence of the synthetic oligonucleotides adjuvant CpG ODN 1826 developed lower antibody titers but when combined to CT, CpG addition resulted in enhanced IgG responses characterized by lower IgG1 levels. Considering the limitations of antigens formulations that can be used in humans, mucosal adjuvants can be a reliable alternative for the development of new strategies of immunization using recombinant proteins of P. vivax. en
dc.format.extent 313-317
dc.language.iso eng
dc.publisher Fundaco Oswaldo Cruz
dc.relation.ispartof Memorias Do Instituto Oswaldo Cruz
dc.rights Acesso aberto
dc.subject Plasmodium vivax en
dc.subject mucosal immunization en
dc.subject recombinant vaccines en
dc.subject adjuvants en
dc.title Adjuvant requirement for successful immunization with recombinant derivatives of Plasmodium vivax merozoite surface protein-1 delivered via the intranasal route en
dc.type Artigo
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.contributor.institution Universidade de São Paulo (USP)
dc.description.affiliation Universidade Federal de São Paulo, Dept Microbiol Imunol Parasitol, Escola Paulista Med, BR-04044010 São Paulo, Brazil
dc.description.affiliation Universidade Federal de São Paulo, Ctr Interdisciplinar Terapia Genica, Escola Paulista Med, BR-04044010 São Paulo, Brazil
dc.description.affiliation Univ São Paulo, Dept Microbiol, Inst Ciencias Biomed, São Paulo, Brazil
dc.description.affiliation Univ São Paulo, Dept Anal Clin & Toxicol, Fac Ciencias Farmaceut, São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Dept Microbiol Imunol Parasitol, Escola Paulista Med, BR-04044010 São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Ctr Interdisciplinar Terapia Genica, Escola Paulista Med, BR-04044010 São Paulo, Brazil
dc.identifier.file S0074-02762007000300010.pdf
dc.identifier.scielo S0074-02762007005000039
dc.identifier.doi 10.1590/S0074-02762007005000039
dc.description.source Web of Science
dc.identifier.wos WOS:000247965700010



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