Adjuvant requirement for successful immunization with recombinant derivatives of Plasmodium vivax merozoite surface protein-1 delivered via the intranasal route

Adjuvant requirement for successful immunization with recombinant derivatives of Plasmodium vivax merozoite surface protein-1 delivered via the intranasal route

Author Bargieri, Daniel Y. Autor UNIFESP Google Scholar
Rosa, Daniela S. Autor UNIFESP Google Scholar
Simoes Lasaro, Melissa Ang Google Scholar
Ferreira, Luis Carlos S. Google Scholar
Soares, Irene S. Google Scholar
Rodrigues, Mauricio M. Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Abstract Recently, we generated two bacterial recombinant proteins expressing 89 amino acids of the C-terminal domain of the Plasmodium vivax merozoite surface protein-1 and the hexa-histidine tag (His6MSP1(19)). One of these recombinant proteins contained also the amino acid sequence of the universal pan allelic T-cell epitope (His(6)MSP1(19)-PADRE). in the present study, we evaluated the immunogenic properties of these antigens when administered via the intra-nasal route in the presence of distinct adjuvant formulations. We found that C57BL/6 mice immunized with either recombinant proteins in the presence of the adjuvants cholera toxin (CT) or the Escherichia coli heat labile toxin ( LT) developed high and long lasting titers of specific serum antibodies. the induced immune responses reached maximum levels after three immunizing doses with a prevailing IgG1 subclass response. in contrast, mice immunized by intranasal route with His(6)MSP1(19)-PADRE in the presence of the synthetic oligonucleotides adjuvant CpG ODN 1826 developed lower antibody titers but when combined to CT, CpG addition resulted in enhanced IgG responses characterized by lower IgG1 levels. Considering the limitations of antigens formulations that can be used in humans, mucosal adjuvants can be a reliable alternative for the development of new strategies of immunization using recombinant proteins of P. vivax.
Keywords Plasmodium vivax
mucosal immunization
recombinant vaccines
adjuvants
Language English
Date 2007-06-01
Published in Memorias Do Instituto Oswaldo Cruz. Rio de Janeiro, Rj: Fundaco Oswaldo Cruz, v. 102, n. 3, p. 313-317, 2007.
ISSN 0074-0276 (Sherpa/Romeo, impact factor)
Publisher Fundaco Oswaldo Cruz
Extent 313-317
Origin http://dx.doi.org/10.1590/S0074-02762007005000039
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000247965700010
SciELO ID S0074-02762007005000039 (statistics in SciELO)
URI http://repositorio.unifesp.br/handle/11600/29778

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