Modulatory effect of killed Propionibacterium acnes and its purified soluble polysaccharide on peritoneal exudate cells from C57Bl/6 mice: Major NKT cell recruitment and increased cytotoxicity

Modulatory effect of killed Propionibacterium acnes and its purified soluble polysaccharide on peritoneal exudate cells from C57Bl/6 mice: Major NKT cell recruitment and increased cytotoxicity

Author Ananias, Renata Zeigler Autor UNIFESP Google Scholar
Rodrigues, E. G. Autor UNIFESP Google Scholar
Braga, E. G. Google Scholar
Squaiella, Carla Cristina Autor UNIFESP Google Scholar
Mussalem, J. S. Google Scholar
Longhini, A. L. F. Google Scholar
Travassos, Luiz Rodolpho Autor UNIFESP Google Scholar
Longo-Maugéri, Ieda Maria Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract Propionibacterium acnes has been described as a potent adjuvant to immune responses in vitro and in vivo. Presently, we analysed the modulation of peritoneal exudate cells (PEC) by heat-killed P. acnes or its purified soluble polysaccharide (PS), both injected intraperitoneally in C57Bl/6 mice, aiming at their recruitment and cytotoxicity. Both treatments induced an increase in macrophages, immature dendritic cells, B1a lymphocytes and NK1.1(+) CD3(+) cells. the bacterium caused a remarkable increase in a NK1.1(+) CD3(+) CD4(-) CD8(-) cells subpopulation, whereas the PS component seemed responsible for the recruitment of mainly macrophage cells. To assess P. acnes and PS adjuvant effect on PEC cytotoxicity we evaluated their in vitro effect on murine B16F10 melanoma cells. the effector cells from the heat-killed bacteria and PS-treated groups lysed melanoma cells in co-cultures with PEC. Mice genetically deficient in IFN-gamma, when stimulated with P. acnes or PS, had reduced PEC cytotoxicity, and the cytotoxic effect was completely abrogated in PEC from iNOS(-/-) mice. the tumoricidal activity of PEC from P. acnes-treated mice was mediated by macrophages and NKT cells stimulated with IL-12. in PS-treated mice the cytotoxicity was mediated mainly by macrophages. Moreover, both treatments increased IL-4 and IFN-gamma production by NKT cells. in conclusion, we show that P. acnes act mainly by recruiting and activating NKT double-negative cells in PEC, which were shown to be tumoricidal in vitro when induced by IL-12. Macrophages induced by both P. acnes and PS have their antitumour effect dependent on NO production.
Language English
Date 2007-06-01
Published in Scandinavian Journal of Immunology. Oxford: Blackwell Publishing, v. 65, n. 6, p. 538-548, 2007.
ISSN 0300-9475 (Sherpa/Romeo, impact factor)
Publisher Blackwell Publishing
Extent 538-548
Origin http://dx.doi.org/10.1111/j.1365-3083.2007.01939.x
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000246713700007
URI http://repositorio.unifesp.br/handle/11600/29768

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