Hyaluronidases in Loxosceles intermedia (brown spider) venom are endo-beta-N-acetyl-D-hexosaminidases hydrolases

Hyaluronidases in Loxosceles intermedia (brown spider) venom are endo-beta-N-acetyl-D-hexosaminidases hydrolases

Author Silveira, Rafael Bertoni da Google Scholar
Chaim, Olga Meiri Google Scholar
Mangili, Oldernir Carlos Google Scholar
Gremski, Waldemiro Google Scholar
Dietrich, Carl Peter Google Scholar
Nader, Helena B. Google Scholar
Veiga, Silvio Sanches Google Scholar
Institution Univ Fed Parana
Universidade Federal de São Paulo (UNIFESP)
Catholic Univ Parana
Abstract In studying Loxosceles venom, we detected degradation of purified hyaluronic acid (HA) and hydrolysis of purified chondroitin sulphate (CS) while neither dermatan sulphate, heparin or heparan sulphate were affected. in addition, with HA-degrading kinetic assays, we show that a hydrolase enzyme was involved in the HA cleavage. By use of the Reissig colorimetric reaction, we found that venom hyaluronidase is an endo-beta-N-acetyl-D-hexosaminidase that generates terminal N-acetylglucosamine residues upon cleavage of HA. Zymogram analysis of L. intermedia venom showed HA lytic activities at 41 and 43 kDa, and, when CS was used as a substrate, zymograph experiments resulted in 41 and 43 kDa lytic zones. Thus, these results support the hypothesis that the same molecules are involved in cleaving HA and CS residues. Experiments to compare L. intermedia electrostimulated venom and venom gland extract also demonstrated very similar HA lytic activity, suggesting again that hyaluronidases are self-components of Loxosceles spider venom instead of oral egesta contamination. HA degradation as a function of pH in these hydrolase enzymes showed no apparent activities at low or high pH, with optimal activity at 6.0-8.0 pH. Finally, we confirmed the cleaving action of the venom hyaluronidases on HA in the extracellular matrix of the dermis of rabbit by fluorescence reaction to HA and confocal microscope analysis. Thus, hyaluronidases type hydrolases endo-beta-N-acetyl-D-hexosaminidase are implicated as self-components of Loxosceles spider venom and can be involved in venom effects as spreading factors. (c) 2006 Elsevier B.V. All rights reserved.
Keywords brown spider
Loxosceles intermedia venom and hyaluronidases
Language English
Date 2007-05-01
Published in Toxicon. Oxford: Pergamon-Elsevier B.V., v. 49, n. 6, p. 758-768, 2007.
ISSN 0041-0101 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 758-768
Origin http://dx.doi.org/10.1016/j.toxicon.2006.11.024
Access rights Closed access
Type Article
Web of Science ID WOS:000246547900002
URI http://repositorio.unifesp.br/handle/11600/29714

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