A role for HO-1 in renal function impairment in animals subjected to ischemic and reperfusion injury and treated with immunosuppressive drugs

A role for HO-1 in renal function impairment in animals subjected to ischemic and reperfusion injury and treated with immunosuppressive drugs

Author Feitoza, C. Q. Google Scholar
Goncalves, G. M. Google Scholar
Bertocchi, A. P. F. Google Scholar
Wang, P. W. Google Scholar
Damiao, M. J. Google Scholar
Cenedeze, M. A. Google Scholar
Teixeira, V. P. A. Google Scholar
Reis, M. A. dos Google Scholar
Pacheco-Silva, A. Google Scholar
Camara, N. O. S. Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Univ Triangulo Mineiro
Universidade de São Paulo (USP)
Abstract Introduction. Ischemia/reperfusion injury (IRI) represents the single major antigenin-dependent factor implicated in pathogenesis of chronic graft dysfunction. Tacrolimus is a calcineurin inhibitor, which has been suggested to be helpful in cyclosporine-related chronic toxicity. Rapamycin has antiproliferative properties that may impair renal regeneration after IRI. Therefore, immunosuppressive drugs might impair renal graft outcome in those organs suffering IRI.Material and Methods. C57B1/6 male mice subjected to 45 minutes of renal pedicle ligation were reperfused for 24 hours. Mice were treated with rapamycin, cyclosporine, or tacrolimus. Blood and renal tissue samples were collected at 24 hours after IRL Urea levels were measured. Heme Oxygenase 1 (HO-1) gene transcript was amplified by a real-time polymerase chain reaction technique.Results. Animals treated with cyclosporine and subjected to IRI showed impaired renal function that peaked at 24 hours. Additional pretreatment with rapamycin produced even more impairment of renal function, when compared with controls. However, tacrolimus pretreatment was associated with a better renal outcome. HO-1 expression was upregulated after IRI by 2.6 arbitrary units at 24 hours. Rapamycin showed worse impairment of renal function.Conclusion. Tacrolimus was not associated with worsening renal function when compared with animals just subjected to IRL Upregulation of HO-1 may be an attractive approach to limit graft injury.
Language English
Date 2007-03-01
Published in Transplantation Proceedings. New York: Elsevier B.V., v. 39, n. 2, p. 424-426, 2007.
ISSN 0041-1345 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 424-426
Origin http://dx.doi.org/10.1016/j.transproceed.2007.01.035
Access rights Closed access
Type Article
Web of Science ID WOS:000245344200029
URI http://repositorio.unifesp.br/handle/11600/29567

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