Purification and partial characterization of two phospholipases A(2) from Bothrops leucurus (white-tailed-jararaca) snake venom

Purification and partial characterization of two phospholipases A(2) from Bothrops leucurus (white-tailed-jararaca) snake venom

Author Higuchi, D. A. Google Scholar
Barbosa, C. M. V. Google Scholar
Bincoletto, C. Google Scholar
Chagas, J. R. Google Scholar
Magalhaes, A. Google Scholar
Richardson, M. Google Scholar
Sanchez, E. F. Google Scholar
Pesquero, J. B. Google Scholar
Araujo, R. C. Google Scholar
Pesquero, J. L. Google Scholar
Institution Univ Mogi Das Cruzes
Universidade Federal de São Paulo (UNIFESP)
Universidade Federal de Minas Gerais (UFMG)
Abstract Two proteins with phospholipase A(2) (PLA(2)) activity were purified to homogeneity from Bothrops leucurus (white-tailed-jararaca) snake venom through three chromatographic steps: Conventional gel filtration on Sephacryl S-200, ion-exchange on Q-Sepharose and reverse phase on Vydac C4 HPLC column. the molecular mass for both enzymes was estimated to be approximately 14 kDa by SDS-PAGE. the N-terminal sequences (48 residues) show that one enzyme presents lysine at position 48 and the other an aspartic acid in this position, and therefore they were designated blK-PLA(2) and blD-PLA(2) respectively. blK-PLA(2) presented negligible levels of PLA(2) activity as compared to that of blD-PLA(2). the PLA(2) activity of both enzymes is Ca2+-dependent. blD-PLA(2) did not have any effect upon platelet aggregation induced by arachidonic acid, ADP or collagen, but strongly inhibits coagulation and is able to stimulate Ehrlich tumor growth but not angiogenesis. (c) 2006 Elsevier Masson SAS. All rights reserved.
Keywords Bothrops leucurus
PLA(2)
platelet aggregation
blood coagulation
angiogenesis
snake venom
Language English
Date 2007-03-01
Published in Biochimie. Paris: Elsevier France-editions Scientifiques Medicales Elsevier, v. 89, n. 3, p. 319-328, 2007.
ISSN 0300-9084 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 319-328
Origin http://dx.doi.org/10.1016/j.biochi.2006.10.010
Access rights Closed access
Type Article
Web of Science ID WOS:000245998900006
URI http://repositorio.unifesp.br/handle/11600/29540

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