Rapid detection and identification of metallo-beta-lactamase-encoding genes by multiplex real-time PCR assay and melt curve analysis

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dc.contributor.author Mendes, Rodrigo E. [UNIFESP]
dc.contributor.author Kiyota, Katia A. [UNIFESP]
dc.contributor.author Monteiro, Jussimara [UNIFESP]
dc.contributor.author Castanheira, Mariana
dc.contributor.author Andrade, Soraya S. [UNIFESP]
dc.contributor.author Gales, Ana C. [UNIFESP]
dc.contributor.author Pignatari, Antonio C. C.
dc.contributor.author Tufik, Sergio [UNIFESP]
dc.date.accessioned 2016-01-24T12:41:50Z
dc.date.available 2016-01-24T12:41:50Z
dc.date.issued 2007-02-01
dc.identifier http://dx.doi.org/10.1128/JCM.01728-06
dc.identifier.citation Journal of Clinical Microbiology. Washington: Amer Soc Microbiology, v. 45, n. 2, p. 544-547, 2007.
dc.identifier.issn 0095-1137
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/29478
dc.description.abstract Metallo-p-lactamase enzymes (MOL) are encoded by transferable genes, which appear to spread rapidly among gram-negative bacteria. the objective of this study was to develop a multiplex real-time PCR assay followed by a melt curve step for rapid detection and identification of genes encoding MOL-type enzymes based on the amplicon melting peak. the reference sequences of all genes encoding IMP and VIM types, SPM-1, GIM-1, and SIM-1 were downloaded from GenBank, and primers were designed to obtain amplicons showing different sizes and melting peak temperatures (T.). the real-time PCR assay was able to detect all M beta L-harboring clinical isolates, and the T-m-assigned genotypes were 100% coincident with previous sequencing results. This assay could be suitable for identification of MOL-producing gram-negative bacteria by molecular diagnostic laboratories. en
dc.format.extent 544-547
dc.language.iso eng
dc.publisher Amer Soc Microbiology
dc.relation.ispartof Journal of Clinical Microbiology
dc.rights Acesso aberto
dc.title Rapid detection and identification of metallo-beta-lactamase-encoding genes by multiplex real-time PCR assay and melt curve analysis en
dc.type Artigo
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.contributor.institution AFIP
dc.description.affiliation Universidade Federal de São Paulo, Div Infect Dis, Lab Especial Microbiol Clin, BR-04025010 São Paulo, Brazil
dc.description.affiliation Universidade Federal de São Paulo, Div Infect Dis, Lab ALERTA, BR-04025010 São Paulo, Brazil
dc.description.affiliation AFIP, Med Lab, São Paulo, Brazil
dc.description.affiliation Universidade Federal de São Paulo, Dept Psychobiol, São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Div Infect Dis, Lab Especial Microbiol Clin, BR-04025010 São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Div Infect Dis, Lab ALERTA, BR-04025010 São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Dept Psychobiol, São Paulo, Brazil
dc.identifier.file WOS000244270000045.pdf
dc.identifier.doi 10.1128/JCM.01728-06
dc.description.source Web of Science
dc.identifier.wos WOS:000244270000045



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