Single multiplex assay to identify simultaneously enteropathogenic, enteroaggregative, enterotoxigenic, enteroinvasive and Shiga toxin-producing Escherichia coli strains in Brazilian children

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dc.contributor.author Aranda, Katia R. S. [UNIFESP]
dc.contributor.author Fabbricotti, Sandra H. [UNIFESP]
dc.contributor.author Fagundes-Neto, Ulysses [UNIFESP]
dc.contributor.author Scaletsky, Isabel C. A. [UNIFESP]
dc.date.accessioned 2016-01-24T12:41:50Z
dc.date.available 2016-01-24T12:41:50Z
dc.date.issued 2007-02-01
dc.identifier http://dx.doi.org/10.1111/j.1574-6968.2006.00580.x
dc.identifier.citation Fems Microbiology Letters. Oxford: Blackwell Publishing, v. 267, n. 2, p. 145-150, 2007.
dc.identifier.issn 0378-1097
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/29474
dc.description.abstract A multiplex PCR to differentiate typical and atypical enteropathogenic Escherichia coli (EPEC), enteroaggregative E. coli (EAEC), enterotoxigenic (ETEC), enteroinvasive E. coli (EIEC) and Shiga toxin-producing E. coli (STEC) strains was developed and evaluated. the targets selected for each group were eae and bfpA for EPEC, aggR for EAEC, elt and est for ETEC, ipaH for EIEC and stx for STEC isolates. This PCR was specific and sensitive for rapid detection of target isolates in stools. Among 79 children with acute diarrhea, this technique identified 13 (16.4%) with atypical EPEC, four (5%) with EAEC, three (3.8%) with typical EPEC, one (1.3%) with ETEC and one (1.3%) with EIEC. en
dc.format.extent 145-150
dc.language.iso eng
dc.publisher Blackwell Publishing
dc.relation.ispartof Fems Microbiology Letters
dc.rights Acesso aberto
dc.subject multiplex PCR en
dc.subject Escherichia coli en
dc.subject diarrhea en
dc.title Single multiplex assay to identify simultaneously enteropathogenic, enteroaggregative, enterotoxigenic, enteroinvasive and Shiga toxin-producing Escherichia coli strains in Brazilian children en
dc.type Artigo
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation Universidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, BR-04023062 São Paulo, Brazil
dc.description.affiliation Universidade Federal de São Paulo, Escola Paulista Med, Dept Pediat, BR-04023062 São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, BR-04023062 São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Escola Paulista Med, Dept Pediat, BR-04023062 São Paulo, Brazil
dc.identifier.doi 10.1111/j.1574-6968.2006.00580.x
dc.description.source Web of Science
dc.identifier.wos WOS:000243506900002



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