Single multiplex assay to identify simultaneously enteropathogenic, enteroaggregative, enterotoxigenic, enteroinvasive and Shiga toxin-producing Escherichia coli strains in Brazilian children

Single multiplex assay to identify simultaneously enteropathogenic, enteroaggregative, enterotoxigenic, enteroinvasive and Shiga toxin-producing Escherichia coli strains in Brazilian children

Author Aranda, Katia R. S. Autor UNIFESP Google Scholar
Fabbricotti, Sandra H. Autor UNIFESP Google Scholar
Fagundes-Neto, Ulysses Autor UNIFESP Google Scholar
Scaletsky, Isabel C. A. Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract A multiplex PCR to differentiate typical and atypical enteropathogenic Escherichia coli (EPEC), enteroaggregative E. coli (EAEC), enterotoxigenic (ETEC), enteroinvasive E. coli (EIEC) and Shiga toxin-producing E. coli (STEC) strains was developed and evaluated. the targets selected for each group were eae and bfpA for EPEC, aggR for EAEC, elt and est for ETEC, ipaH for EIEC and stx for STEC isolates. This PCR was specific and sensitive for rapid detection of target isolates in stools. Among 79 children with acute diarrhea, this technique identified 13 (16.4%) with atypical EPEC, four (5%) with EAEC, three (3.8%) with typical EPEC, one (1.3%) with ETEC and one (1.3%) with EIEC.
Keywords multiplex PCR
Escherichia coli
diarrhea
Language English
Date 2007-02-01
Published in Fems Microbiology Letters. Oxford: Blackwell Publishing, v. 267, n. 2, p. 145-150, 2007.
ISSN 0378-1097 (Sherpa/Romeo, impact factor)
Publisher Blackwell Publishing
Extent 145-150
Origin http://dx.doi.org/10.1111/j.1574-6968.2006.00580.x
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000243506900002
URI http://repositorio.unifesp.br/handle/11600/29474

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