HIV-1-Infected children on HAART: Immunologic features of three different levels of viral suppression

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dc.contributor.author Zaccarelli Filho, Carlos Alberto [UNIFESP]
dc.contributor.author Ono, Erika [UNIFESP]
dc.contributor.author Machado, Daisy Maria [UNIFESP]
dc.contributor.author Brunialti, Milena Karina Coló [UNIFESP]
dc.contributor.author Succi, Regina Célia de Menezes [UNIFESP]
dc.contributor.author Salomão, Reinaldo [UNIFESP]
dc.contributor.author Kallas, Esper Georges [UNIFESP]
dc.contributor.author De Moraes-Pinto, Maria Isabel [UNIFESP]
dc.date.accessioned 2016-01-24T12:41:49Z
dc.date.available 2016-01-24T12:41:49Z
dc.date.issued 2007-01-15
dc.identifier http://dx.doi.org/10.1002/cyto.b.20152
dc.identifier.citation Cytometry Part B-clinical Cytometry. Hoboken: Wiley-liss, v. 72B, n. 1, p. 14-21, 2007.
dc.identifier.issn 1552-4949
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/29456
dc.description.abstract Background: HIV-1-infected children show changes of blood lymphocyte subpopulations. We have, therefore, investigated how highly active anti-retroviral therapy (ART) alter these subsets. Blood samples were taken from 41 HIV-1-infected children on ART who were divided into groups showing good, partial and poor responses to ART on the basis of viral load (VL) measurement in blood. the observations were compared to those seen in 20 uninfected children.Methods: the samples were studied using 4-color flow cytometry for naive, central memory and effector memory cells as well as for CD38 expression as the sign of activation within both the CD4+ and the CD8+ T cell populations. HIV-1 infected children were also evaluated for the presence and the titers of antibodies induced by vaccination against childhood infections in our patients while on HAART.Results: Lymphocyte counts were lower in the poor viral load responding (VLR) group when compared with partial and good VLRs. Poor VLRs had lower total and naive CD4+ T cell counts. HIV-1-infected children from all three groups had high CD8+ T cell counts. Central memory CD4+ and CD8+ T cell percentages were particularly low in the poor VLR group while in the poor VLR group the percentages of effector memory CD4+ and CD8+ T cells were higher when compared with the control group. Higher cellular activation of CD8+ T cells was observed in HIV-1-infected children, particularly when analyzed for the intensity of CD38 expression in the poor VLR group. CD5 expression on B cells was higher among all HIV-1-infected children. Antibodies to tetanus, diphtheria, measles, rubella, and hepatitis B were present in a large proportion of children but the titers were similarly low for all three groups of HIV-infected children.Conclusions: Children with different levels of viral response to HAART present immune phenotype characteristics that tend to place the children with partial and good virological responses into the same group. These children are still moderately deficient in their immune responses but show better recovery than seen with children in the poor VLR group. These observations indicate that the proportions of central memory cells among the CD4+ T cells and the intensity of the expression of CD38 activation antigen on CD8+ T cells provide more informative parameters for monitoring children on HAART than the absolute numbers of CD4+ and CD8+ T cells alone. (c) 2006 Clinical Cytometry Society. en
dc.format.extent 14-21
dc.language.iso eng
dc.publisher Wiley-Blackwell
dc.relation.ispartof Cytometry Part B-clinical Cytometry
dc.rights Acesso aberto
dc.subject HIV-infected children en
dc.subject antiretroviral therapy en
dc.subject immune reconstitution en
dc.title HIV-1-Infected children on HAART: Immunologic features of three different levels of viral suppression en
dc.type Artigo
dc.rights.license http://olabout.wiley.com/WileyCDA/Section/id-406071.html
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation Universidade Federal de São Paulo, Res Lab, Div Pediat Infect Dis, BR-04039032 São Paulo, SP, Brazil
dc.description.affiliation Universidade Federal de São Paulo, Div Infect Dis, São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Res Lab, Div Pediat Infect Dis, BR-04039032 São Paulo, SP, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Div Infect Dis, São Paulo, Brazil
dc.identifier.doi 10.1002/cyto.b.20152
dc.description.source Web of Science
dc.identifier.wos WOS:000243322000004



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