Role of distinct immune components in the radiation-induced abrogation of systemic lupus erythematosus development in mice

Role of distinct immune components in the radiation-induced abrogation of systemic lupus erythematosus development in mice

Author Brito, R. R. N. e Autor UNIFESP Google Scholar
De Lorenzo, B. H. P. Autor UNIFESP Google Scholar
Xander, Patricia Autor UNIFESP Google Scholar
Godoy, L. C. Google Scholar
Lopes, J. D. Autor UNIFESP Google Scholar
Silva, N. P. da Autor UNIFESP Google Scholar
Sampaio, S. C. Google Scholar
Mariano, M. Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Univ Massachusetts
Inst Butantan
Abstract The New Zealand Black X New Zealand White F1 [(NZB/NZW) F1] mouse develops an autoimmune condition resembling aspects of human systemic lupus erythematosus (SLE). We investigated the effects of a novel prophylactic thoraco-abdominal gamma irradiation protocol on the onset and evolution of lupus in these animals. Survival of irradiated mice was higher when compared with nonirradiated mice. Kidney lesions were milder and autoantibody levels were lower in irradiated mice. To identify possible mechanisms involved in the radiation-induced improvement of disease, distinct components of humoral and cellular immune responses were evaluated. Because B-1 cells are known to be involved in various autoimmune diseases, we investigated the participation of these cells in SLE progression. Unexpectedly, B-1 cells were not depleted in (NZB/NZW) F1, even after several rounds of irradiation. No alterations were found in viability and physiology of B-1 cells in SLE animals with the exception of constitutive overexpression of the anti-apoptotic molecule Bcl-2, which may account for the observed radioresistance. Thus, a role for B-1 cells in murine SLE cannot be excluded, since the irradiation protocol did not effectively eliminate these cells. Additionally, we demonstrate a marked delay in the ability of splenocytes to repopulate the spleen after irradiation in (NZB/NZW) F1, in contrast to leucocytes in other cellular compartments. the implications of these findings for the fate of SLE in this model are discussed.
Keywords B-1 cells
irradiation
systemic jupus erythematosus
Language English
Date 2007-01-01
Published in Lupus. London: Sage Publications Ltd, v. 16, n. 12, p. 947-954, 2007.
ISSN 0961-2033 (Sherpa/Romeo, impact factor)
Publisher Sage Publications Ltd
Extent 947-954
Origin http://dx.doi.org/10.1177/0961203307084298
Access rights Closed access
Type Article
Web of Science ID WOS:000252200400004
URI http://repositorio.unifesp.br/handle/11600/29447

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