Optical anisotropy of alcian blue-stained acid glycosaminoglycans

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dc.contributor.author Mello, Maria Luiza S.
dc.contributor.author Michelacci, Yara M.
dc.contributor.author Stockert, Juan Carlos
dc.contributor.author Vidal, Benedicto de Campos
dc.date.accessioned 2016-01-24T12:41:43Z
dc.date.available 2016-01-24T12:41:43Z
dc.date.issued 2007-01-01
dc.identifier http://dx.doi.org/10.1016/j.acthis.2006.06.008
dc.identifier.citation Acta Histochemica. Jena: Elsevier Gmbh, Urban & Fischer Verlag, v. 109, n. 1, p. 78-85, 2007.
dc.identifier.issn 0065-1281
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/29369
dc.description.abstract Optical anisotropy as dispersion of birefringence (DB) (birefringence studied for light of different wavelengths) and linear dichroism (LD) (selective absorption of polarized Light) in stained substrates reflects their macromolecular orientation states. Birefringence interference colors of alcian blue (AB)-stained glycosamino-glycans (GAG) and glycoconjugates observed with polarization microscopy have been found to vary, although their staining characteristics under unpolarized Light are practically the same. We investigated the optical anisotropy of GAG-AB and some glycoconjugate-AB complexes used as standards, to provide a basis for interpreting results for AB-stained materials in situ. Filamentous preparations of hyaluronic acid (HA), chondroitinsulfates, proteoglycans, and a mucus sulfoglycoconjugate were studied. Anomalous DB (birefringence sign changing with the wavelength of the incident Light) was generally observed, but LD was seen only in the AB-HA complex. LD simultaneous to anomalous DB characteristics on the AB-HA complex were assumed to be caused by a maximally oriented helical conformation of the HA. for the other AB-GAG studied, the optical anisotropic characteristics were suggestive of some degree of folding of their chains into a tertiary structure. the profiles of the anomalous DB curves for the AB-stained sulfoglycoconjugate differed from those of the other materials, probably due to different organization of its dye-binding sites. (C) 2006 Elsevier GmbH. All rights reserved. en
dc.format.extent 78-85
dc.language.iso eng
dc.publisher Elsevier B.V.
dc.relation.ispartof Acta Histochemica
dc.rights Acesso restrito
dc.subject acid glycosaminoglycans en
dc.subject alcian blue en
dc.subject dispersion of birefringence en
dc.subject linear dichroism en
dc.subject macromolecular orientation en
dc.subject optical anisotropy en
dc.title Optical anisotropy of alcian blue-stained acid glycosaminoglycans en
dc.type Artigo
dc.rights.license http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.contributor.institution Universidade Estadual de Campinas (UNICAMP)
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.contributor.institution Autonomous Univ Madrid
dc.description.affiliation Univ Estadual Campinas, UNICAMP, Inst Biol, Dept Cell Biol, BR-13083863 Campinas, SP, Brazil
dc.description.affiliation Universidade Federal de São Paulo, Dept Biochem, BR-04044020 São Paulo, Brazil
dc.description.affiliation Autonomous Univ Madrid, Dept Biol, E-28049 Madrid, Spain
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Dept Biochem, BR-04044020 São Paulo, Brazil
dc.identifier.doi 10.1016/j.acthis.2006.06.008
dc.description.source Web of Science
dc.identifier.wos WOS:000244594300008



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