Optical anisotropy of alcian blue-stained acid glycosaminoglycans

Optical anisotropy of alcian blue-stained acid glycosaminoglycans

Author Mello, Maria Luiza S. Google Scholar
Michelacci, Yara M. Google Scholar
Stockert, Juan Carlos Google Scholar
Vidal, Benedicto de Campos Google Scholar
Institution Universidade Estadual de Campinas (UNICAMP)
Universidade Federal de São Paulo (UNIFESP)
Autonomous Univ Madrid
Abstract Optical anisotropy as dispersion of birefringence (DB) (birefringence studied for light of different wavelengths) and linear dichroism (LD) (selective absorption of polarized Light) in stained substrates reflects their macromolecular orientation states. Birefringence interference colors of alcian blue (AB)-stained glycosamino-glycans (GAG) and glycoconjugates observed with polarization microscopy have been found to vary, although their staining characteristics under unpolarized Light are practically the same. We investigated the optical anisotropy of GAG-AB and some glycoconjugate-AB complexes used as standards, to provide a basis for interpreting results for AB-stained materials in situ. Filamentous preparations of hyaluronic acid (HA), chondroitinsulfates, proteoglycans, and a mucus sulfoglycoconjugate were studied. Anomalous DB (birefringence sign changing with the wavelength of the incident Light) was generally observed, but LD was seen only in the AB-HA complex. LD simultaneous to anomalous DB characteristics on the AB-HA complex were assumed to be caused by a maximally oriented helical conformation of the HA. for the other AB-GAG studied, the optical anisotropic characteristics were suggestive of some degree of folding of their chains into a tertiary structure. the profiles of the anomalous DB curves for the AB-stained sulfoglycoconjugate differed from those of the other materials, probably due to different organization of its dye-binding sites. (C) 2006 Elsevier GmbH. All rights reserved.
Keywords acid glycosaminoglycans
alcian blue
dispersion of birefringence
linear dichroism
macromolecular orientation
optical anisotropy
Language English
Date 2007-01-01
Published in Acta Histochemica. Jena: Elsevier Gmbh, Urban & Fischer Verlag, v. 109, n. 1, p. 78-85, 2007.
ISSN 0065-1281 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 78-85
Origin http://dx.doi.org/10.1016/j.acthis.2006.06.008
Access rights Closed access
Type Article
Web of Science ID WOS:000244594300008
URI http://repositorio.unifesp.br/handle/11600/29369

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