Imaging malaria sporozoites in the dermis of the mammalian host

Show simple item record Amino, Rogerio [UNIFESP] Thiberge, Sabine Blazquez, Samantha Baldacci, Patricia Renaud, Olivier Shorte, Spencer Menard, Robert 2016-01-24T12:41:41Z 2016-01-24T12:41:41Z 2007-01-01
dc.identifier.citation Nature Protocols. London: Nature Publishing Group, v. 2, n. 7, p. 1705-1712, 2007.
dc.identifier.issn 1754-2189
dc.description.abstract The initial phase of malaria infection is the pre-erythrocytic phase, which begins when parasites are injected by the mosquito into the dermis and ends when parasites are released from hepatocytes into the blood. We present here a protocol for the in vivo imaging of GFP-expressing sporozoites in the dermis of rodents, using the combination of a high-speed spinning-disk confocal microscope and a high-speed charge-coupled device (CCD) camera permitting rapid in vivo acquisitions. the steps of this protocol indicate how to infect mice through the bite of infected Anopheles stephensi mosquitoes, record the sporozoites' fate in the mouse ear and to present the data as maximum-fluorescence-intensity projections, time-lapse representations and movie clips. This protocol permits investigating the various aspects of sporozoite behavior in a quantitative manner, such as motility in the matrix, cell traversal, crossing the endothelial barrier of both blood and lymphatic vessels and intravascular gliding. Applied to genetically modified parasites and/or mice, these imaging techniques should be useful for studying the cellular and molecular bases of Plasmodium sporozoite infection in vivo. en
dc.format.extent 1705-1712
dc.language.iso eng
dc.publisher Nature Publishing Group
dc.relation.ispartof Nature Protocols
dc.rights Acesso aberto
dc.title Imaging malaria sporozoites in the dermis of the mammalian host en
dc.type Artigo
dc.contributor.institution Inst Pasteur
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation Inst Pasteur, Unite Biol & Genet Paludisme, F-75724 Paris 15, France
dc.identifier.doi 10.1038/nprot.2007.120
dc.description.source Web of Science
dc.identifier.wos WOS:000253139200013


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