Imaging malaria sporozoites in the dermis of the mammalian host

Imaging malaria sporozoites in the dermis of the mammalian host

Author Amino, Rogerio Autor UNIFESP Google Scholar
Thiberge, Sabine Google Scholar
Blazquez, Samantha Google Scholar
Baldacci, Patricia Google Scholar
Renaud, Olivier Google Scholar
Shorte, Spencer Google Scholar
Menard, Robert Google Scholar
Institution Inst Pasteur
Universidade Federal de São Paulo (UNIFESP)
Abstract The initial phase of malaria infection is the pre-erythrocytic phase, which begins when parasites are injected by the mosquito into the dermis and ends when parasites are released from hepatocytes into the blood. We present here a protocol for the in vivo imaging of GFP-expressing sporozoites in the dermis of rodents, using the combination of a high-speed spinning-disk confocal microscope and a high-speed charge-coupled device (CCD) camera permitting rapid in vivo acquisitions. the steps of this protocol indicate how to infect mice through the bite of infected Anopheles stephensi mosquitoes, record the sporozoites' fate in the mouse ear and to present the data as maximum-fluorescence-intensity projections, time-lapse representations and movie clips. This protocol permits investigating the various aspects of sporozoite behavior in a quantitative manner, such as motility in the matrix, cell traversal, crossing the endothelial barrier of both blood and lymphatic vessels and intravascular gliding. Applied to genetically modified parasites and/or mice, these imaging techniques should be useful for studying the cellular and molecular bases of Plasmodium sporozoite infection in vivo.
Language English
Date 2007-01-01
Published in Nature Protocols. London: Nature Publishing Group, v. 2, n. 7, p. 1705-1712, 2007.
ISSN 1754-2189 (Sherpa/Romeo, impact factor)
Publisher Nature Publishing Group
Extent 1705-1712
Origin http://dx.doi.org/10.1038/nprot.2007.120
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000253139200013
URI http://repositorio.unifesp.br/handle/11600/29335

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