Ultrastructural morphometric analysis of ameloblasts exposed to fluoride during tooth development

Ultrastructural morphometric analysis of ameloblasts exposed to fluoride during tooth development

Author Ribeiro, Daniel A. Google Scholar
Hirota, Luciane Google Scholar
Cestari, Tania M. Google Scholar
Ceolin, Daniele S. Google Scholar
Taga, Rumio Google Scholar
Assis, Gerson F. Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Abstract Since a considerable amount of the world population is exposed to high doses of fluoride, it is of special concern to investigate its action mechanisms during dental enamel development. in this study, the toxicity of fluoride in ameloblasts during enamel development was evaluated by means of ultrastructural morphometric analysis. A total of 18 male Wistar rats were distributed into three groups. in Group I, the animals received deionized drinking water ad libitum (negative control) and in Groups II and III, they received sodium fluorided (NaF) drinking water at doses of 7 and 100 ppm ad libitum, respectively, for 6 weeks. Morphometric data were expressed as volume density of the most significant organelles present in the secretory and maturation phases of amelogenesis such as RER, granules, lysosomes, phagic vacuoles, microfilaments and mitochondria. the results showed that the volume density of mitochondria in the 100 ppm experimental group was 29% (P < 0.05) higher than the control group in secretory ameloblasts. No remarkable differences were found in maturation ameloblasts for all organelles evaluated. Taken together, these data indicate that NaF at high doses is able to induce cellular damage in secretory ameloblasts, whereas no noxious effect was observed during maturation stage of amelogenesis as depicted by ultrastructural analysis.
Keywords dental fluorosis
fluoride
ameloblast
rat incisor
ultrastructure
Language English
Date 2006-11-01
Published in Journal of Molecular Histology. Dordrecht: Springer, v. 37, n. 8-9, p. 361-367, 2006.
ISSN 1567-2379 (Sherpa/Romeo, impact factor)
Publisher Springer
Extent 361-367
Origin http://dx.doi.org/10.1007/s10735-006-9065-2
Access rights Closed access
Type Article
Web of Science ID WOS:000242435700006
URI http://repositorio.unifesp.br/handle/11600/29225

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