Gene frequencies of the HNA-4a and -5a neutrophil antigens in Brazilian persons and a new polymerase chain reaction-restriction fragment length polymorphism method for HNA-5a genotyping

Gene frequencies of the HNA-4a and -5a neutrophil antigens in Brazilian persons and a new polymerase chain reaction-restriction fragment length polymorphism method for HNA-5a genotyping

Author Braz Cardone, Jose Daniel Google Scholar
Bordin, Jose Orlando Google Scholar
Chiba, Akemi Kuroda Google Scholar
Matico Ikeda Norcia, Angela Maria Google Scholar
Botelho Vieira-Filho, Joao Paulo Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract BACKGROUND: the HNA-4a (Mart) and HNA-5a (Ond) antigens are polymorphic variants of alpha(M) (CD11b) and alpha(L) (CD11a) subunits of the beta(2)-integrin, and are associated with single nucleotide polymorphisms (SNP) leading to amino acid dimorphisms. HNA-4a has been linked to alloimmune neonatal neutropenia, but the HNA-5a clinical significance is unclear.STUDY DESIGN and METHODS: Using a polymerase chain reaction (PCR) with sequence-specific primers, the frequency of HNA-4a among 121 Brazilian blood donors and 114 Amazon Indians was determined. A PCR-restriction fragment length polymorphism (RFLP) method for HNA-5a genotyping was developed, and the gene frequencies of this antigen were determined among 123 blood donors and 114 Indians. To validate the genotyping method, the amplified DNA from six previously obtained samples (two of each genotype) was sequenced.RESULTS: the HNA-4a (+/+), HNA-4a (+/-), and HNA-4a (-/-) genotype frequencies of blood donors (0.686, 0.273, 0.041) and Indians (1.000, 0.000, 0.000) were different (p < 0.01). the frequencies of HNA-5a (+/+), HNA-5a (+/-), and HNA-5a (-/-) genotypes among blood donors (0.512, 0.399, 0.089) and Indians (0.746, 0.219, 0.035) also differed (p < 0.01). Sequencing demonstrated concordance with PCR-RFLP genotyping in all six evaluated samples.CONCLUSION: Comparing to another populations, Brazilians present a higher frequency of HNA-4a-negative allele, suggesting that Brazilians would be more susceptible to HNA-4a alloimmunization. Moreover, the distribution of the HNA-4 alleles observed in Amazon Indians is quite similar to that reported among Koreans. Besides that, a new effective and efficient HNA-5a genotyping technique is now available for population studies.
Language English
Date 2006-09-01
Published in Transfusion. Oxford: Blackwell Publishing, v. 46, n. 9, p. 1515-1520, 2006.
ISSN 0041-1132 (Sherpa/Romeo, impact factor)
Publisher Blackwell Publishing
Extent 1515-1520
Origin http://dx.doi.org/10.1111/j.1537-2995.2006.00943.x
Access rights Closed access
Type Article
Web of Science ID WOS:000240147000011
URI http://repositorio.unifesp.br/handle/11600/29149

Show full item record




File

File Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Search


Browse

Statistics

My Account