Effect of primer selection on estimates of GB virus C (GBV-C) prevalence and response to antiretroviral therapy for optimal testing for GBV-C viremia

Effect of primer selection on estimates of GB virus C (GBV-C) prevalence and response to antiretroviral therapy for optimal testing for GBV-C viremia

Author Souza, I. E. Autor UNIFESP Google Scholar
Allen, J. B. Google Scholar
Xiang, J. Google Scholar
Klinzman, D. Google Scholar
Diaz, R. Google Scholar
Zhang, S. Google Scholar
Chaloner, K. Google Scholar
Zdunek, D. Google Scholar
Hess, G. Google Scholar
Williams, C. F. Google Scholar
Benning, L. Google Scholar
Stapleton, J. T. Google Scholar
Institution Univ Iowa
Iowa City Vet Adm Med Ctr
Universidade Federal de São Paulo (UNIFESP)
Roche Diagnost GmbH
NIAID
Johns Hopkins Univ
Abstract GB virus C (GBV-C; also called hepatitis G virus) is a common cause of infection associated with prolonged survival among HIV-infected individuals. the prevalences of GBV-C viremia vary widely in different studies, and there has been poor agreement among different laboratories performing GBV-C RNA detection in quality control studies. To determine the optimal method of measuring GBV-C RNA in clinical samples, samples obtained from 939 HIV-infected subjects were studied using reverse transcription (RT)-PCR methods amplifying four separate regions of the GBV-C genome. Primers amplifying the E2 coding region were 100% specific; however, their sensitivity was only 76.6%. in contrast, primers amplifying three additional conserved regions of the GBV-C genome (the 5' nontranslated region and the nonstructural protein-coding regions 3 and 5A) were more sensitive but produced higher rates of false-positive results. Using low-specificity primer sets influenced the significance of association between GBV-C viremia and response to antiretroviral therapy. Using a quantitative GBV-C RNA method, the GBV-C RNA concentration did not correlate with baseline or set point HIV RNA levels; however, a correlation between negative, low, and high GBV-C RNA levels and increasing reduction in HIV RNA following antiretroviral therapy was observed. Subjects with both GBV-C E2 antibody and viremia had significantly lower GBV-C RNA levels than did viremic subjects without E2 antibody. These studies demonstrate that accurate detection of GBV-C RNA by nested RT-PCR requires the use of primers representing multiple genome regions. Analyses based on testing with single primers do not lead to reliable conclusions about the association between GBV-C infection and clinical outcomes.
Language English
Date 2006-09-01
Published in Journal of Clinical Microbiology. Washington: Amer Soc Microbiology, v. 44, n. 9, p. 3105-3113, 2006.
ISSN 0095-1137 (Sherpa/Romeo, impact factor)
Publisher Amer Soc Microbiology
Extent 3105-3113
Origin http://dx.doi.org/10.1128/JCM.02663-05
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000240708000007
URI http://repositorio.unifesp.br/handle/11600/29113

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