Cells positive for microtubule-associated protein 1B (MAP 1B) are present along rat and human efferent ductules and epididymis

Cells positive for microtubule-associated protein 1B (MAP 1B) are present along rat and human efferent ductules and epididymis

Autor Queiroz, DBC Google Scholar
Silva, A. M. da Google Scholar
Gutierrez-Ospina, G. Google Scholar
Porto, C. S. Google Scholar
Grossman, G. Google Scholar
Petrusz, P. Google Scholar
Avellar, MCW Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Natl Autonomous Univ Mexico
Univ N Carolina
Resumo Microtubule-associated protein 1B (MAP 1B) is a neuronal cytoskeleton marker with predominant expression in the developing nervous system. the present study provides evidence for the expression of this cytoskeleton protein in non-neuronal and neuronal cells along rat and human efferent ductules and epididymis (initial segment, caput, and cauda). Reverse transcription/polymerase chain reaction and Western blot analysis were used to confirm the presence of MAP 1B (mRNA and protein) in rat tissues. Immunohistochemical studies revealed MAP-1B-positive staining in columnar ciliated cells present in efferent ductules and in narrow cells located in the initial segment, in both rat and human. MAP-1B-positive basal cells, located underneath the columnar cells, were only identified in the initial segment and caput epididymidis of the rat. Qualitative analysis of tissues from 40-day-old and 120-day-old rats indicated that the number of MAP-1B-positive ciliated, narrow, and basal cells per tubule increased with sexual maturation. These immunoreactive cells did not stain for dopamine beta-hydroxylase or acetylcholinesterase, indicating that they were not adrenergic or cholinergic in nature. Immunohistochemical studies also revealed the presence of MAP-1B-positive staining in interstitial nerve fibers in caput and cauda epididymidis from both rat and human. Thus, the expression of MAP 1B is not confined to a specific cell type in rat and human efferent ductules and epididymis. the functional significance of this cytoskeleton protein in tissues from the male reproductive tract requires further investigation.
Assunto epididymis
efferent ductules
microtubule-associated protein
innervation
male reproductive tract
rat (Wistar)
human
Idioma Inglês
Data 2006-07-01
Publicado em Cell and Tissue Research. New York: Springer, v. 325, n. 1, p. 125-133, 2006.
ISSN 0302-766X (Sherpa/Romeo, fator de impacto)
Editor Springer
Extensão 125-133
Fonte http://dx.doi.org/10.1007/s00441-005-0108-x
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000238317000013
URI http://repositorio.unifesp.br/handle/11600/29002

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