Human colostrum contains IgA antibodies reactive to colonization factors I and II of enterotoxigenic Escherichia coli

Human colostrum contains IgA antibodies reactive to colonization factors I and II of enterotoxigenic Escherichia coli

Author Correa, Simone Google Scholar
Palmeira, Patricia Google Scholar
Carneiro-Sampaio, Magda Maria Sales Google Scholar
Nishimura, Lucília Sanae Autor UNIFESP Google Scholar
Guth, Beatriz Ernestina Cabilio Autor UNIFESP Google Scholar
Institution Inst Ciencias Biomed
Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Abstract Diarrhea is an important cause of morbidity and mortality amongst infants of low socio-economic levels in developing countries and in travelers who visit such areas. Enterotoxigenic E. coli strains express two sets of virulence-associated factors: enterotoxins (heat-stable toxins or heat-labile toxins) and colonization factors. Studies have shown that breast-feeding protects infants against infectious diseases, such as diarrhea, as it presents a great variety of immunological components. the aim of this study was to analyze the reactivity of immunoglobulin A from human colostrum to colonization factor antigens I and II. the colostrum ability in preventing enterotoxigenic E. coli adhesion to Caco-2 cells was also evaluated. Colostrum samples were collected from 32 healthy women, and a human colostrum. pool was prepared. Enterotoxigenic E. coli strains expressing colonization factor antigens I and 11 were utilized. the colostrum pool and individual samples showed variable antienterotoxigenic E. coli immunoglobulin A titers, that were reactive with colonization factor antigen I and CS1/CS3 (colonization factor antigen 11). the human colostrum pool and individual samples inhibited enterotoxigenic E coli colonization factor antigen I and II adhesion to Caco-2 cells, at variable levels, and this ability was a result of immunoglobulin A antibodies reactive to these colonization factors. the immunoglobulin A-depleted pool lost this inhibitory ability. As bacterial adhesion is the initial mechanism of enterotoxigenic E. coli infection, breast-feeding could protect the offspring against diarrhea caused by this agent.
Keywords enterotoxigenic
Escherichia coli
secretory IgA
colonization factor antigen I
colonization factor antigen II
human colostrum
Language English
Date 2006-07-01
Published in Fems Immunology and Medical Microbiology. Oxford: Blackwell Publishing, v. 47, n. 2, p. 199-206, 2006.
ISSN 0928-8244 (Sherpa/Romeo, impact factor)
Publisher Blackwell Publishing
Extent 199-206
Origin http://dx.doi.org/10.1111/j.1574-695X.2006.00082.x
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000238761500005
URI http://repositorio.unifesp.br/handle/11600/28992

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