The effect of brown spider venom on endothelial cell morphology and adhesive structures

The effect of brown spider venom on endothelial cell morphology and adhesive structures

Author Paludo, Katia Sabrina Google Scholar
Gremski, Luiza Helena Google Scholar
Veiga, Silvio Sanches Google Scholar
Chaim, Olga Meiri Google Scholar
Gremski, Waldemiro Google Scholar
Freitas Buchi, Dorly de Google Scholar
Nader, Helena Bonciani Google Scholar
Dietrich, Carl Peter Google Scholar
Franco, Celia Regina Cavichiolo Autor UNIFESP Google Scholar
Institution Univ Fed Parana
Universidade Federal de São Paulo (UNIFESP)
Catholic Univ Parana
Abstract Spiders of the Loxosceles genus have been responsible for severe clinical cases of envenomation worldwide. Accidents involving brown spiders can cause dermonecrotic injury, hemorrhage, hemolysis, platelet aggregation and renal failure. Histological findings of animals treated by venom have shown subendothelial blebs, vacuoles and endothelial cell membrane degeneration of blood vessel walls, as well as fibrin and thrombus formation. the mechanisms by which the venom causes these disorders are poorly understood. in this work, with an endothelial cell line derived from rabbit aorta, we were able to demonstrate that venom binds to the cell surface and the extracellular matrix. Moreover, we observed that the venom also induced morphological alterations, such as cell retraction, homophilic disadhesion and an increasing in filopodia projections. We also demonstrated that toxins present in the venom disorganized focal adhesion points and actin microfilaments of endothelial cells. Nevertheless, endothelial cell viability showed no alterations compared to controls. Additionally, venom treatment changed the fibronectin matrix profile synthesized by these cells as well as cell adhesion to fibronectin. These results suggest that the deleterious effects of venom on blood vessel walls could be a consequence of the direct effect on the endothelial cell surface and adhesive structures involved in blood vessel stability. These effects indirectly lead to leukocyte and platelet activation, disseminated intravascular coagulation and an increase in vessel permeability. (c) 2006 Elsevier B.V. All rights reserved.
Keywords brown spider
venom
cell adhesion
cytoskeleton
extracellular matrix
Language English
Date 2006-06-15
Published in Toxicon. Oxford: Pergamon-Elsevier B.V., v. 47, n. 8, p. 844-853, 2006.
ISSN 0041-0101 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 844-853
Origin http://dx.doi.org/10.1016/j.toxicon.2006.02.006
Access rights Closed access
Type Article
Web of Science ID WOS:000238962800003
URI http://repositorio.unifesp.br/handle/11600/28980

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