Direct electron paramagnetic resonance monitoring of the peptide synthesis coupling reaction in polymeric support

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dc.contributor.author Nakaie, Clovis R.
dc.contributor.author Malavolta, Luciana
dc.contributor.author Schreier, Shirley
dc.contributor.author Trovatti, Eliane
dc.contributor.author Marchetto, Reinaldo
dc.date.accessioned 2016-01-24T12:41:15Z
dc.date.available 2016-01-24T12:41:15Z
dc.date.issued 2006-06-14
dc.identifier http://dx.doi.org/10.1016/j.polymer.2006.05.007
dc.identifier.citation Polymer. Oxford: Elsevier B.V., v. 47, n. 13, p. 4531-4536, 2006.
dc.identifier.issn 0032-3861
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/28976
dc.description.abstract This work demonstrates, for the first time. a time-resolved electron paramagnetic resonance (EPR) monitoring of a chemical reaction occurring in a polymeric structure. the progress of the coupling of a N-alpha-tert-butyloxycarbonyl-2.2.6.6-tetramethylpiperidine-1-oxyl-4-amino-4-carboxylic acid (Boc-TOAC) spin probe to a model peptide-resin was followed through EPR spectra. Progressive line broadening of EPR peaks was observed, indicative of an increased population of immobilized spin probe molecules attached to the solid support. the time for spectral stabilization of this process coincided with that determined in a previous Coupling study. thereby validating this in situ quantitative monitoring of the reaction. in addition, the influence of polymer swelling degree and solvent viscosity, as well as of the steric hindrance within beads. on the rate of coupling reaction was also addressed. A deeper evaluation of the latter effect was possible by determining unusual polymer parameters such as the average site-site distance and site-concentration within resin beads in each solvent system. (c) 2006 Elsevier B.V. All rights reserved. en
dc.format.extent 4531-4536
dc.language.iso eng
dc.publisher Elsevier B.V.
dc.relation.ispartof Polymer
dc.rights Acesso restrito
dc.subject peptide en
dc.subject polymer en
dc.subject electron paramagnetic resonance en
dc.title Direct electron paramagnetic resonance monitoring of the peptide synthesis coupling reaction in polymeric support en
dc.type Artigo
dc.rights.license http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.contributor.institution Universidade de São Paulo (USP)
dc.contributor.institution UNESP
dc.description.affiliation Universidade Federal de São Paulo, Dept Biophys, BR-04044020 São Paulo, Brazil
dc.description.affiliation Univ São Paulo, Inst Chem, Dept Biochem, BR-05513970 São Paulo, Brazil
dc.description.affiliation UNESP, Dept Biochem & Technol Chem, Inst Chem, BR-14800900 São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Dept Biophys, BR-04044020 São Paulo, Brazil
dc.identifier.doi 10.1016/j.polymer.2006.05.007
dc.description.source Web of Science
dc.identifier.wos WOS:000238921300010



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