Please use this identifier to cite or link to this item: http://repositorio.unifesp.br/handle/11600/28976
Title: Direct electron paramagnetic resonance monitoring of the peptide synthesis coupling reaction in polymeric support
Authors: Nakaie, Clovis R.
Malavolta, Luciana
Schreier, Shirley
Trovatti, Eliane
Marchetto, Reinaldo
Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
UNESP
Keywords: peptide
polymer
electron paramagnetic resonance
Issue Date: 14-Jun-2006
Publisher: Elsevier B.V.
Citation: Polymer. Oxford: Elsevier B.V., v. 47, n. 13, p. 4531-4536, 2006.
Abstract: This work demonstrates, for the first time. a time-resolved electron paramagnetic resonance (EPR) monitoring of a chemical reaction occurring in a polymeric structure. the progress of the coupling of a N-alpha-tert-butyloxycarbonyl-2.2.6.6-tetramethylpiperidine-1-oxyl-4-amino-4-carboxylic acid (Boc-TOAC) spin probe to a model peptide-resin was followed through EPR spectra. Progressive line broadening of EPR peaks was observed, indicative of an increased population of immobilized spin probe molecules attached to the solid support. the time for spectral stabilization of this process coincided with that determined in a previous Coupling study. thereby validating this in situ quantitative monitoring of the reaction. in addition, the influence of polymer swelling degree and solvent viscosity, as well as of the steric hindrance within beads. on the rate of coupling reaction was also addressed. A deeper evaluation of the latter effect was possible by determining unusual polymer parameters such as the average site-site distance and site-concentration within resin beads in each solvent system. (c) 2006 Elsevier B.V. All rights reserved.
URI: http://repositorio.unifesp.br/handle/11600/28976
ISSN: 0032-3861
Other Identifiers: http://dx.doi.org/10.1016/j.polymer.2006.05.007
Appears in Collections:Em verificação - Geral

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.