Trypanosoma cruzi disrupts myofibrillar organization and intracellular calcium levels in mouse neonatal cardiomyocytes

Trypanosoma cruzi disrupts myofibrillar organization and intracellular calcium levels in mouse neonatal cardiomyocytes

Autor Taniwaki, N. N. Google Scholar
Machado, F. S. Google Scholar
Massensini, A. R. Google Scholar
Mortara, R. A. Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Inst Adolofo Lutz
Universidade de São Paulo (USP)
Universidade Federal de Minas Gerais (UFMG)
Resumo Immunofluorescence studies of normal and Trypanosoma cruzi-infected primary cultures of heart muscle cells were performed to gather information about the arrangement of myofibrillar components during the intracellular life cycle of this parasite. By using a panel of monoclonal antibodies against various myofibrillar proteins, a progressive disruption and loss of contractile proteins (such myosin and actin) of the host cell was detected during infection. the host cell formed a loose network of myofibrillar proteins around the parasites. Breakdown of the myofibrils occurred in regions where the parasites were present, and heavily infected cells showed myofibrillar proteins at their periphery. in parallel, we investigated the effect of T. cruzi infection on intracellular calcium levels by using a Ca(2+) fluorescent indicator (confocal microscopy). Infected cardiomyocytes displayed a marked impairment in contractility, and calcium influxes became irregular and less intense when compared with those of non-infected cells. Our results demonstrate that T. cruzi infection dramatically affects calcium fluxes and causes myofibrillar breakdown disturbing cardiomyocyte contractility.
Palavra-chave cardiomyocytes
calcium
myofibrils
monoclonal antibodies
confocal microscopy
Trypanosoma cruzi
cell culture (Mouse)
Idioma Inglês
Data de publicação 2006-06-01
Publicado em Cell and Tissue Research. New York: Springer, v. 324, n. 3, p. 489-496, 2006.
ISSN 0302-766X (Sherpa/Romeo, fator de impacto)
Publicador Springer
Extensão 489-496
Fonte http://dx.doi.org/10.1007/s00441-005-0152-6
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000237121300015
Endereço permanente http://repositorio.unifesp.br/handle/11600/28941

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