Diagnosis of suspicious thyroid nodules using four protein biomarkers

Diagnosis of suspicious thyroid nodules using four protein biomarkers

Autor Cerutti, Janete M. Google Scholar
Latini, Flavia RM Google Scholar
Nakabashi, Claudia Google Scholar
Delcelo, Rosana Autor UNIFESP Google Scholar
Andrade, Victor P. Google Scholar
Amadei, Marcelo Joao Google Scholar
Maciel, Rui MB Google Scholar
Hojaij, Flavio Carneiro Autor UNIFESP Google Scholar
Hollis, Donna Google Scholar
Shoemaker, Jennifer Google Scholar
Riggins, Gregory J. Google Scholar
Instituição Johns Hopkins Univ
Universidade Federal de São Paulo (UNIFESP)
Duke Univ
Resumo Purpose: Fine-needle aspiration (FNA) cytology, a standard method for thyroid nodule diagnosis, cannot distinguish between benign follicular thyroid adenoma (FTA) and malignant follicular thyroid carcinoma (FTC). Previously, using expression profiling, we found that a combination of transcript expression levels from DDIT3, ARGZ C1orf24, and ITM1 distinguished between FTA and FTC. the goal of this study was to determine if antibody markers used alone or in combination could accurately distinguish between a wider variety of benign and malignant thyroid lesions in fixed sections and FNA samples.Experimental Design: Immunohistochemistry was done on 27 FTA, 25 FTC, and 75 other benign and malignant thyroid tissue sections using custom antibodies for chromosome 1 open reading frame 24 (C1orf24) and integral membrane protein 1 (ITM1) and commercial antibodies for DNA damage - inducible transcript 3 (DDIT3) and arginase II (ARG2). FNA samples were also tested using the same antibodies. RNA expression was measured by quantitative PCR in 33 thyroid lesions.Results: C1orf24 and ITM1 antibodies had an estimated sensitivity of 1.00 for distinguishing FTA from FTC. for the expanded analysis of all lesions studied, ITM1 had an estimated sensitivity of 1.00 for detecting malignancy. Because all four cancer biomarkers did well, producing overlapping confidence intervals, not one best marker was distinguished. Transcript levels also reliably predicted malignancy, but immunohistochemistry had a higher sensitivity. Malignant cells were easily detected in FNA samples using these markers.Conclusions: We improved this diagnostic test by adding C1orf24 and ITM1 custom antibodies and showing use on a wider variety of thyroid pathology. We recommend that testing of all four cancer biomarkers now be advanced to larger trials. Use of one or more of these antibodies should improve diagnostic accuracy of suspicious thyroid nodules from both tissue sections and FNA samples.
Idioma Inglês
Data de publicação 2006-06-01
Publicado em Clinical Cancer Research. Philadelphia: Amer Assoc Cancer Research, v. 12, n. 11, p. 3311-3318, 2006.
ISSN 1078-0432 (Sherpa/Romeo, fator de impacto)
Publicador Amer Assoc Cancer Research
Extensão 3311-3318
Fonte http://dx.doi.org/10.1158/1078-0432.CCR-05-2226
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000238169800013
Endereço permanente http://repositorio.unifesp.br/handle/11600/28931

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