The substrate specificity of cruzipain 2, a cysteine protease isoform from Trypanosoma cruzi

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dc.contributor.author Reis, Flavia Coelho Garcia dos
dc.contributor.author Judice, Wagner AS
dc.contributor.author Juliano, Maria Aparecida [UNIFESP]
dc.contributor.author Juliano, Luiz [UNIFESP]
dc.contributor.author Scharfstein, Julio
dc.contributor.author Lima, Ana Paula CD
dc.date.accessioned 2016-01-24T12:41:12Z
dc.date.available 2016-01-24T12:41:12Z
dc.date.issued 2006-06-01
dc.identifier http://dx.doi.org/10.1111/j.1574-6968.2006.00267.x
dc.identifier.citation Fems Microbiology Letters. Oxford: Blackwell Publishing, v. 259, n. 2, p. 215-220, 2006.
dc.identifier.issn 0378-1097
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/28927
dc.description.abstract Papain-like cysteine proteases are important for the survival of the flagellated protozoa Trypanosoma cruzi, the causative agent of Chagas' Disease. the lysosomal cysteine protease designated as cruzipain or cruzain, is the archetype of a multigene family of related isoforms. We investigated the substrate specificity of the cruzipain 2 isoform using internally quenched fluorogenic substrates. We found that cruzipain 2 and cruzain differ substantially regarding the specificity in the S-2, S-1(') and S-2(') pockets. Our study indicates that cruzipain 2 has a more restricted specificity than cruzain, suggesting that these isoforms might act on distinct natural substrates. en
dc.format.extent 215-220
dc.language.iso eng
dc.publisher Blackwell Publishing
dc.relation.ispartof Fems Microbiology Letters
dc.rights Acesso aberto
dc.subject cysteine protease en
dc.subject trypanosome en
dc.subject Chagas Disease en
dc.subject specificity en
dc.subject isoform en
dc.title The substrate specificity of cruzipain 2, a cysteine protease isoform from Trypanosoma cruzi en
dc.type Artigo
dc.contributor.institution Universidade Federal do Rio de Janeiro (UFRJ)
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation Univ Fed Rio de Janeiro, Inst Biofis Carlos Chagas Filho, BR-21949900 Rio de Janeiro, Brazil
dc.description.affiliation Universidade Federal de São Paulo, Escola Paulista Med, São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Escola Paulista Med, São Paulo, Brazil
dc.identifier.doi 10.1111/j.1574-6968.2006.00267.x
dc.description.source Web of Science
dc.identifier.wos WOS:000237739500008



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