The substrate specificity of cruzipain 2, a cysteine protease isoform from Trypanosoma cruzi

The substrate specificity of cruzipain 2, a cysteine protease isoform from Trypanosoma cruzi

Author Reis, Flavia Coelho Garcia dos Google Scholar
Judice, Wagner AS Google Scholar
Juliano, Maria Aparecida Autor UNIFESP Google Scholar
Juliano, Luiz Autor UNIFESP Google Scholar
Scharfstein, Julio Google Scholar
Lima, Ana Paula CD Google Scholar
Institution Universidade Federal do Rio de Janeiro (UFRJ)
Universidade Federal de São Paulo (UNIFESP)
Abstract Papain-like cysteine proteases are important for the survival of the flagellated protozoa Trypanosoma cruzi, the causative agent of Chagas' Disease. the lysosomal cysteine protease designated as cruzipain or cruzain, is the archetype of a multigene family of related isoforms. We investigated the substrate specificity of the cruzipain 2 isoform using internally quenched fluorogenic substrates. We found that cruzipain 2 and cruzain differ substantially regarding the specificity in the S-2, S-1(') and S-2(') pockets. Our study indicates that cruzipain 2 has a more restricted specificity than cruzain, suggesting that these isoforms might act on distinct natural substrates.
Keywords cysteine protease
trypanosome
Chagas Disease
specificity
isoform
Language English
Date 2006-06-01
Published in Fems Microbiology Letters. Oxford: Blackwell Publishing, v. 259, n. 2, p. 215-220, 2006.
ISSN 0378-1097 (Sherpa/Romeo, impact factor)
Publisher Blackwell Publishing
Extent 215-220
Origin http://dx.doi.org/10.1111/j.1574-6968.2006.00267.x
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000237739500008
URI http://repositorio.unifesp.br/handle/11600/28927

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