A novel category of enteropathogenic Escherichia coli simultaneously utilizes the Nck and TccP pathways to induce actin remodelling

A novel category of enteropathogenic Escherichia coli simultaneously utilizes the Nck and TccP pathways to induce actin remodelling

Autor Whale, Andrew D. Google Scholar
Garmendia, Junkal Google Scholar
Gomes, Tania A. Autor UNIFESP Google Scholar
Frankel, Gad Google Scholar
Instituição Univ London Imperial Coll Sci Technol & Med
Universidade Federal de São Paulo (UNIFESP)
Resumo Enterohaemorrhagic Escherichia coli (EHEC) and enteropathogenic E. coli (EPEC) induce drastic reorganization of the microfilament cytoskeleton. EHEC and EPEC translocate Tir (translocated intimin receptor) which, once inserted into the host plasma membrane, binds the bacterial outer membrane adhesin intimin. Tir(EPEC) then becomes tyrosine phosphorylated facilitating the recruitment and site-specific binding of the eukaryotic adaptor Nck, which in turn binds and activates the Wiskott-Aldrich syndrome protein (N-WASP), leading to actin-related protein 2/3 (Arp2/3) complex-mediated actin polymerization. in contrast, Tir(EHEC) has no Nck binding site; instead, EHEC utilizes the translocated effector TccP (Tir-cytoskeleton coupling protein) to bind and activate N-WASP. Here we report a novel class of EPEC that translocates both TccP and Tir(EPEC)-like effector molecules. Consistent with these characteristics, we show that both the Tir-Nck and Tir:TccP actin remodelling pathways function simultaneously during infection, making this a novel and versatile EPEC category.
Idioma Inglês
Data de publicação 2006-06-01
Publicado em Cellular Microbiology. Oxford: Blackwell Publishing, v. 8, n. 6, p. 999-1008, 2006.
ISSN 1462-5814 (Sherpa/Romeo, fator de impacto)
Publicador Blackwell Publishing
Extensão 999-1008
Fonte http://dx.doi.org/10.1111/j.1462-5822.2006.00682.x
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000237394900009
Endereço permanente http://repositorio.unifesp.br/handle/11600/28925

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