Pro-epileptic effect of alfentanil in rats subjected to pilocarpine-induced chronic epilepsy

Pro-epileptic effect of alfentanil in rats subjected to pilocarpine-induced chronic epilepsy

Autor Garrido Sanabria, Emilio Rafael Google Scholar
D'Andrea Vieira, Isabella Google Scholar
Fernanda Da Silveira Pereira, Maria Google Scholar
Coutinho Faria, Leonardo Google Scholar
Da Silva, Andre Cesar Google Scholar
Abrao Cavalheiro, Esper Google Scholar
Silva Fernandes, Maria Jose da Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Univ Brownsville
Resumo Pharmacological induction of epileptiform activity is a complementary method to study the epileptogenic area in drug-resistant epileptic patients. Among the different activation methods, fentanyl derivatives (e.g. alfentanil) provide one of the most efficient tools in triggering epileptiform abnormalities in surgical candidates. in this study, we tested the pro-epileptic effect of different concentrations of alfentanil in hippocampal slices obtained from control and pilocarpine-treated chronic epileptic rats. the pro-convulsant action of alfentanil was also studied in control and pilocarpine-treated epileptic rats implanted with subdural and hippocampal electrodes for electroencephalographic recordings. in 90% of slices from control animals, application of affentanil (0.1-5 mu M) induced a significant enhancement in amplitude and number of population spikes recorded in the hippocampal CA1 region. in contrast, alfentanil produced a significant reduction in the amplitude of population spikes in slices from pilocarpine-treated epileptic rats. These changes were accompanied by a significant increase in the number of population spikes in the form of epileptiform multispike responses of epileptic slices. Naloxone (20 mu M) antagonized the effect of alfentanil in both control and epileptic slices, reducing the number of population spikes in slices from epileptic rats. in control rats, alfentanil induced epileptiform abnormalities in the hippocampal and cortical electroencephalographic recordings but only at concentrations higher than 200 [mu g/kg (e.g. 350 mu g/kg). Lower doses of affentanil (25 mu g/kg) elicited epileptiform abnormalities only in chronic epileptic rats. the potent action of a minimal dose of alfentanil in inducing epileptiform activity suggests an enhancement of the pro-convulsant action of mu-receptor opioids in chronic temporal lobe epilepsy. (c) 2006 Elsevier Inc. All rights reserved.
Palavra-chave opioid
fentanyl
seizures
hippocampus
naloxone
temporal lobe epilepsy
Idioma Inglês
Data de publicação 2006-05-15
Publicado em Brain Research Bulletin. Oxford: Pergamon-Elsevier B.V., v. 69, n. 5, p. 535-545, 2006.
ISSN 0361-9230 (Sherpa/Romeo, fator de impacto)
Publicador Elsevier B.V.
Extensão 535-545
Fonte http://dx.doi.org/10.1016/j.brainresbull.2006.02.016
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000237678000007
Endereço permanente http://repositorio.unifesp.br/handle/11600/28909

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