Hepatoblastomas and liver development: A study of cytokeratin immunoexpression in twenty-nine hepatoblastomas

Hepatoblastomas and liver development: A study of cytokeratin immunoexpression in twenty-nine hepatoblastomas

Autor Cajaiba, Mariana Morais Google Scholar
Neves, Jose Ivanildo Google Scholar
Casarotti, Fabiana Facco Google Scholar
De Camargo, Beatriz Google Scholar
Chapchap, Paulo Google Scholar
Sredni, Simone Treiger Google Scholar
Soares, Fernando Augusto Google Scholar
Instituição Hosp Canc AC Camargo São Paulo Brazil
Universidade Federal de São Paulo (UNIFESP)
Resumo Hepatoblastomas (HBs) recapitulate liver development. It is possible that HBs result from malignant transformation of hepatic precursor cells, and they may reflect a blockage in normal development. Here we study the expression of cytokeratins (CKs) in order to delineate the immunoprofile and relationship with liver development, as well as vimentin and alphafetoprotein (AFP), of HBs. Immunohistochemistry was performed in a tissue microarray (TMA) containing representative areas of 18 HBs (fetal and/or embryonal and/or mesenchymal); we also reviewed I I cases not included in the TMA. No cases stained for CKs 1, 5/6, 7, 10, 13, 15, 16, 20, and 34 beta E12. CK8 stained 73.07% of fetal, 50% of embryonal, and 18% of mesenchymal areas. CK18 stained 100% of epithelial areas. CK19 staining was intense and diffuse in 100% of embryonal samples, but it was weaker in fetal areas (66.66%). AE1 stained epithelial areas in all cases, and it stained 29.41% of mesenchymal areas. AE3 stained 84.61% of embryonal and 60% of fetal components. AE1/AE3 showed stronger staining in embryonal (100%) than in fetal areas (76.92%). Vimentin staining was strong in embryonal (66.66%) and mesenchymal (84.61%) components but weak in fetal areas (8%). Alphafetoprotein was positive in only 20% of fetal and 70% of embryonal areas. Our results support the hypothesis that immunoexpression of HBs follows the stages of normal liver development. Embryonal areas look less differentiated, expressing vimentin and biliary epithelium CKs, whereas fetal areas display a
Palavra-chave cytokeratins
development
hepatoblastomas
immunohistochemistry
tissue microarray
Idioma Inglês
Data de publicação 2006-05-01
Publicado em Pediatric and Developmental Pathology. Lawrence: Alliance Communications Group Division Allen Press, v. 9, n. 3, p. 196-202, 2006.
ISSN 1093-5266 (Sherpa/Romeo, fator de impacto)
Publicador Alliance Communications Group Division Allen Press
Extensão 196-202
Fonte http://dx.doi.org/10.2350/05-12-0002.1
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000248595900004
Endereço permanente http://repositorio.unifesp.br/handle/11600/28908

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