Universal Collection Medium (UCM)(R) is as suitable as the Standard Transport Medium (STM)(R) for Hybrid Capture II (R) (HC-2) assay

Universal Collection Medium (UCM)(R) is as suitable as the Standard Transport Medium (STM)(R) for Hybrid Capture II (R) (HC-2) assay

Autor Taha, NSA Google Scholar
Focchi, J. Google Scholar
Ribalta, JCL Google Scholar
Castelo, A. Google Scholar
Lorincz, A. Google Scholar
Dores, G. B. Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Digene USA
Resumo Background: Collection of cervical-vaginal material in liquid media enables simultaneous evaluation of both oncologic cytology and molecular tests for the detection of Human papillomavirus (HPV), Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (NG). Universal Collection Medium(R) (UCM) has been developed to fulfill this objective.Objectives: To compare Hybrid Capture II(R) (HC-2) to diagnose HPV, NG and CT in specimens collected in UCM(R) and in the current Digene Standard Transport Medium(R) (STM).Study design: the study was cross-sectional. Three collections of endocervical and ectocervical material were performed in each of 893 women referred for colposcopy in the following order: (1) to prepare a conventional Pap smear slide using the accompanying brush of the STM kit and with Ayre spatula; (2) for HC-2 test and liquid-based cytology using a 1 ml UCM vial as transport medium; material was collected with another similar brush; (3) for HC-2 test using a I ml STM vial as transport medium, material was collected with the same brush that we used in the procedure no. (1) (conventional Pap smear). HC-2 results from samples taken from STM and UCM media were compared by using simple linear regression analysis and Kappa statistic.Results and conclusions: HC-2 results from the two media were highly correlated: high-risk HPV (kappa = 0.92 r(2) =0.92), low-risk HPV (kappa = 0.85; r(2) = 0.86) and NG/CT (kappa = 0.96; r(2) = 0.81). Despite being obtained from a second specimen, the UCM HC-2 results were equivalent to those obtained with the standard medium STM and the UCM medium. (C) 2006 Elsevier B.V. All rights reserved.
Palavra-chave UCM
STM
HPV
CT
NG
diagnosis
Idioma Inglês
Data de publicação 2006-05-01
Publicado em Journal of Clinical Virology. Amsterdam: Elsevier B.V., v. 36, n. 1, p. 32-35, 2006.
ISSN 1386-6532 (Sherpa/Romeo, fator de impacto)
Publicador Elsevier B.V.
Extensão 32-35
Fonte http://dx.doi.org/10.1016/j.jcv.2005.12.011
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000237127700006
Endereço permanente http://repositorio.unifesp.br/handle/11600/28885

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