Mycobacterium hsp65 DNA entrapped into TDM-loaded PLGA microspheres induces protection in mice against Leishmania (Leishmania) major infection

Mycobacterium hsp65 DNA entrapped into TDM-loaded PLGA microspheres induces protection in mice against Leishmania (Leishmania) major infection

Author Coelho, EAF Google Scholar
Tavares, CAP Google Scholar
Lima, K. D. Google Scholar
Silva, C. L. Google Scholar
Rodrigues, J. M. Google Scholar
Fernandes, A. P. Google Scholar
Institution Universidade Federal de Minas Gerais (UFMG)
Universidade Federal de São Paulo (UNIFESP)
Nanocore Biotecnol Ltda
Abstract Heat shock proteins (HSPs) are highly conserved among different organisms. A mycobacterial HSP65 DNA vaccine was previously shown to have prophylactic and immunotherapeutic effects against Mycobacterium tuberculosis infection in mice. Here, BALB/c mice were immunized with mycobacterial DNA-hsp65 or with DNA-hsp65 and trehalose dymicolate (TDM), both carried by biodegradable microspheres (MHSP/TDM), and challenged with Leishmania (Leishmania) major. MHSP/TDM conferred protection against L. major infection, as indicated by a significant reduction of edema and parasite loads in infected tissues. Although high levels of interferon-gamma and low levels of interleukin (IL)-4 and IL-10 were detected in mice immunized with DNA-hsp65 or MHSP/TDM, only animals immunized with MHSP/TDM displayed a consistent Th1 immune response, i.e., significantly higher levels of anti-soluble Leishmania antigen (SLA) immunoglobulin G (IgG)2a and low anti-SLA IgG1 antibodies. These findings indicate that encapsulated MHSP/TDM is more immunogenic than naked hsp65 DNA, and has great potential to improve vaccine effectiveness against leishmaniasis and tuberculosis.
Language English
Date 2006-05-01
Published in Parasitology Research. New York: Springer, v. 98, n. 6, p. 568-575, 2006.
ISSN 0932-0113 (Sherpa/Romeo, impact factor)
Publisher Springer
Extent 568-575
Origin http://dx.doi.org/10.1007/s00436-005-0088-5
Access rights Closed access
Type Article
Web of Science ID WOS:000236499000012
URI http://repositorio.unifesp.br/handle/11600/28877

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