The effect of a selective cyclooxygenase-2 (COX-2) inhibitor on the proliferation rate of retinoblastoma cell lines

The effect of a selective cyclooxygenase-2 (COX-2) inhibitor on the proliferation rate of retinoblastoma cell lines

Autor Souza, J. P. de Google Scholar
Corrêa, Zélia Maria da Silva Autor UNIFESP Google Scholar
Marshall, J. C. Google Scholar
Anteka, E. Google Scholar
Coutinho, A. B. Google Scholar
Martins, M. C. Google Scholar
Burnier, M. N. Google Scholar
Instituição McGill Univ
Universidade Federal de São Paulo (UNIFESP)
Retina & Oncol Serv
Resumo Purpose To examine the effect of nepafenac, a selective cyclooxygenase-2 (COX-2) inhibitor, on the proliferation rate of two human retinoblastoma (Rb) cell lines.Methods Two human Rb cell lines (WERI-RB and Y79) were cultured. COX-2 expression in these cell lines was verified by imunocytochemical analysis of cytospin sections and Western blotting. An MTT-based proliferation assay was used to compare Rb cell growth with and without amfenac, the active metabolite of nepafenac. the averaged results per condition were recorded. the Student's t-test was used to compare results from the cells cultured with and without amfenac.Result the Y79 cell line showed a higher proliferative rate than the WERI-RB cell line. Both cell lines were negative for COX-2 expression by immunocytochemical analysis; however, both cell lines were positive for COX-2 expression by Western blot. When amfenac was added to both of the cell lines, a statistically significant reduction in proliferation was observed in both cell lines. the two Rb cell lines were positive for COX-2 only in the Western blot, indicating that they probably express low levels of COX-2, which was undetectable by immucytochemical analysis.Conclusion the selective, anti-COX-2 molecule amfenac inhibited proliferation of both tested Rb cell lines. Further trials should be undertaken to study the effect of selective COX-2 inhibitors on Rb.
Palavra-chave COX-2
retinoblastoma
nepafenac
cell lines
immunocytochemistry
Idioma Inglês
Data de publicação 2006-05-01
Publicado em Eye. London: Nature Publishing Group, v. 20, n. 5, p. 598-601, 2006.
ISSN 0950-222X (Sherpa/Romeo, fator de impacto)
Publicador Nature Publishing Group
Extensão 598-601
Fonte http://dx.doi.org/10.1038/sj.eye.6701938
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000237360000016
Endereço permanente http://repositorio.unifesp.br/handle/11600/28853

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