Purification of a phospholipase A(2) from Lonomia obliqua caterpillar bristle extract

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dc.contributor.author Seibert, C. S.
dc.contributor.author Tanaka-Azevedo, A. M.
dc.contributor.author Santoro, M. L.
dc.contributor.author Mackessy, S. P.
dc.contributor.author Torquato, RJS
dc.contributor.author Lebrun, I
dc.contributor.author Tanaka, A. S.
dc.contributor.author Sano-Martins, I. S.
dc.date.accessioned 2016-01-24T12:41:07Z
dc.date.available 2016-01-24T12:41:07Z
dc.date.issued 2006-04-21
dc.identifier http://dx.doi.org/10.1016/j.bbrc.2006.02.071
dc.identifier.citation Biochemical and Biophysical Research Communications. San Diego: Academic Press Inc Elsevier Science, v. 342, n. 4, p. 1027-1033, 2006.
dc.identifier.issn 0006-291X
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/28851
dc.description.abstract Lonomia obliqua caterpillar bristle extract induces both direct and indirect hemolytic activity on human and rat washed erythrocytes, and provokes intravascular hemolysis in Wistar rats. Indirect hemolytic activity is assumed to be caused by a phospholipase A(2) (PLA(2)) present in this extract, and this investigation was initiated in order to characterize this enzyme. Phospholipase A, activity of crude extract was inhibited by both a PLA(2)-specific inhibitor (pBpb) and the metal ion chelator EDTA. L. obliqua PLA(2) was purified by liquid chromatography from the crude bristle extract and had a molecular mass of 15 kDa and a pI of 5.9: its N-terminal sequence showed high homology to a sequence of a putative PLA(2) obtained from a cDNA library of L. obliqua bristles, and it is tentatively placed among Group III phospholipases A(2). This enzyme was stable at 4 degrees C sensitive to higher temperatures, and its maximum catalytic activity was at pH 8.0. L. obliqua PLA(2) induced hemolysis only when incubated with exogenous lecithin. Thus, the PLA(2) purified herein appears to be responsible for the indirect hemolytic activity of the crude bristle extract. (c) 2006 Elsevier Inc. All rights reserved. en
dc.format.extent 1027-1033
dc.language.iso eng
dc.publisher Elsevier B.V.
dc.relation.ispartof Biochemical and Biophysical Research Communications
dc.rights Acesso restrito
dc.subject Lonomia obliqua en
dc.subject phospholipase A(2) en
dc.subject indirect hemolysis en
dc.subject erythrocytes en
dc.subject intravascular hemolysis en
dc.subject caterpillar en
dc.subject lecithin en
dc.subject chromatography en
dc.title Purification of a phospholipase A(2) from Lonomia obliqua caterpillar bristle extract en
dc.type Artigo
dc.rights.license http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.contributor.institution Inst Butanan
dc.contributor.institution Univ No Colorado
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation Inst Butanan, Lab Fisiopatol, São Paulo, Brazil
dc.description.affiliation Univ No Colorado, Sch Biol Sci, Greeley, CO 80639 USA
dc.description.affiliation UNIFESP, Dept Bioquim, São Paulo, Brazil
dc.description.affiliation Inst Butanan, Lab Bioquim & Biofis, São Paulo, Brazil
dc.description.affiliationUnifesp UNIFESP, Dept Bioquim, São Paulo, Brazil
dc.identifier.doi 10.1016/j.bbrc.2006.02.071
dc.description.source Web of Science
dc.identifier.wos WOS:000236316100004



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