Purification of a phospholipase A(2) from Lonomia obliqua caterpillar bristle extract

Purification of a phospholipase A(2) from Lonomia obliqua caterpillar bristle extract

Autor Seibert, C. S. Google Scholar
Tanaka-Azevedo, A. M. Google Scholar
Santoro, M. L. Google Scholar
Mackessy, S. P. Google Scholar
Torquato, RJS Google Scholar
Lebrun, I Google Scholar
Tanaka, A. S. Google Scholar
Sano-Martins, I. S. Google Scholar
Instituição Inst Butanan
Univ No Colorado
Universidade Federal de São Paulo (UNIFESP)
Resumo Lonomia obliqua caterpillar bristle extract induces both direct and indirect hemolytic activity on human and rat washed erythrocytes, and provokes intravascular hemolysis in Wistar rats. Indirect hemolytic activity is assumed to be caused by a phospholipase A(2) (PLA(2)) present in this extract, and this investigation was initiated in order to characterize this enzyme. Phospholipase A, activity of crude extract was inhibited by both a PLA(2)-specific inhibitor (pBpb) and the metal ion chelator EDTA. L. obliqua PLA(2) was purified by liquid chromatography from the crude bristle extract and had a molecular mass of 15 kDa and a pI of 5.9: its N-terminal sequence showed high homology to a sequence of a putative PLA(2) obtained from a cDNA library of L. obliqua bristles, and it is tentatively placed among Group III phospholipases A(2). This enzyme was stable at 4 degrees C sensitive to higher temperatures, and its maximum catalytic activity was at pH 8.0. L. obliqua PLA(2) induced hemolysis only when incubated with exogenous lecithin. Thus, the PLA(2) purified herein appears to be responsible for the indirect hemolytic activity of the crude bristle extract. (c) 2006 Elsevier Inc. All rights reserved.
Palavra-chave Lonomia obliqua
phospholipase A(2)
indirect hemolysis
erythrocytes
intravascular hemolysis
caterpillar
lecithin
chromatography
Idioma Inglês
Data de publicação 2006-04-21
Publicado em Biochemical and Biophysical Research Communications. San Diego: Academic Press Inc Elsevier Science, v. 342, n. 4, p. 1027-1033, 2006.
ISSN 0006-291X (Sherpa/Romeo, fator de impacto)
Publicador Elsevier B.V.
Extensão 1027-1033
Fonte http://dx.doi.org/10.1016/j.bbrc.2006.02.071
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000236316100004
Endereço permanente http://repositorio.unifesp.br/handle/11600/28851

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