Recombinant interleukin-4-treated macrophages, epithelioid cell surrogates, harbor and arrest Mycobacterium avium multiplication in vitro

Recombinant interleukin-4-treated macrophages, epithelioid cell surrogates, harbor and arrest Mycobacterium avium multiplication in vitro

Autor Chinen, LTD Google Scholar
Cipriano, N. M. Google Scholar
Oliveira, R. S. de Google Scholar
Leao, S. C. Google Scholar
Mariano, M. Google Scholar
Carneiro, CRW Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Discipline Dev Biol
Resumo Our group has previously described that murine peritoneal macrophages treated in vitro for 7 days with recombinant interleukin-4 (rIL-4) acquire morphological and functional characteristics of epithelioid cells (ECs) found in granulomatous lesions. Although EC function has not been clarified so far, it has been suggested that these cells could present antigens and control multiplication of mycobacteria. These aspects have been addressed here using in vitro EC surrogates. Using immunocytochemistry and immunofluorescence methods, we have observed an increased expression of CD11b, CD54, CD86 and CD40 molecules on rIL-4-treated macrophages when compared to untreated ones. Cytokine-treated cells were less phagocytic for latex beads (P < 0.03) and more pinocytic for dextran particles than untreated macrophages. T-cell lymphoproliferation assays using ovalbumin (OVA) and Mycobacterium avium as antigens showed that both cultured macrophages were equally efficient as antigen presenting cells (APCs). However, M. avium antigens were better presented in vivo by EC surrogates (P < 0.01). Both macrophage cultures were similarly infected by M. avium. However, while the infection level was maintained in the cytokine-treated population, untreated macrophages showed a progressive increase in the number of bacilli/cell with time (P < 0.01) and a reduction of about 65% in cell population. After 96 h of M. avium infection, untreated cells secreted higher amounts of tumor necrosis factor-alpha (P < 0.005) while rIL-4-treated macrophages showed higher, although not significant, transforming growth factor-beta production. Also, EC surrogates produced less nitric oxide than control macrophages (P < 0.05). Hence, EC surrogates restrain M. avium growth and act as APCs in vitro and in vivo. (c) 2006 Elsevier SAS. All rights reserved.
Palavra-chave epithelioid cells
macrophages
recombinant interleukin-4
Mycobacterium avium
Idioma Inglês
Data de publicação 2006-04-01
Publicado em Microbes and Infection. Amsterdam: Elsevier B.V., v. 8, n. 4, p. 965-973, 2006.
ISSN 1286-4579 (Sherpa/Romeo, fator de impacto)
Publicador Elsevier B.V.
Extensão 965-973
Fonte http://dx.doi.org/10.1016/j.micinf.2005.09.017
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000237754100002
Endereço permanente http://repositorio.unifesp.br/handle/11600/28820

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