An IncI1 plasmid contributes to the adherence of the atypical enteroaggregative Escherichia coli strain C1096 to cultured cells and abiotic surfaces

An IncI1 plasmid contributes to the adherence of the atypical enteroaggregative Escherichia coli strain C1096 to cultured cells and abiotic surfaces

Autor Dudley, Edward G. Google Scholar
Abe, Cecilia Mari Autor UNIFESP Google Scholar
Ghigo, Jean Marc Google Scholar
Latour-Lambert, Patricia Google Scholar
Hormazabal, Juan Carlos Google Scholar
Nataro, James P. Google Scholar
Instituição Univ Maryland
Universidade Federal de São Paulo (UNIFESP)
Inst Pasteur
Inst Salud Publ Chile
Resumo Enteroaggregative Escherichia coli (EAEC) is defined by a characteristic stacked-brick aggregative adherence (AA) pattern to cultured cells. in well-studied EAEC prototype strains (called typical EAEC strains), the AA phenotype requires aggregative adherence fimbriae (AAFs). However, previous studies suggest that known AAF alleles are not found in all EAEC strains. To define mechanisms contributing to adherence in an atypical strain, we studied EAEC strain C1096. An E. coli K12 derivative carrying two plasmids, designated pSERB1 and pSERB2, from C1096 adhered to cell lines and exhibited an AA pattern. Nucleotide sequence analysis of pSERB1 indicated that it is related to plasmids of the IncI1 incompatibility group. These plasmids encode genes involved in pilus-mediated conjugal transfer, as well as pilL-V, which encodes a second pilus of the type IV family. Insertional inactivation of the gene predicted to encode the major type IV pilin subunit (pilS) reduced conjugal transfer of the plasmid by 4 orders of magnitude. Adherence of the mutant strain to polystyrene and to HT29 cells was reduced by approximately 21% and 75%, respectively. in a continuous-flow microfermentor, the pilS inactivation reduced mature biofilm formation on a glass slide by approximately 50%. in addition, the simultaneous presence of both pSERB1 and pSERB2 plasmids promoted pilS-independent biofilm formation. We conclude that the IncI1 plasmid of EAEC C1096 encodes a type IV pilus that contributes to plasmid conjugation, epithelial cell adherence, and adherence to abiotic surfaces. We also observe that AA can be mediated by factors distinct from AAF adhesins.
Idioma Inglês
Data de publicação 2006-04-01
Publicado em Infection and Immunity. Washington: Amer Soc Microbiology, v. 74, n. 4, p. 2102-2114, 2006.
ISSN 0019-9567 (Sherpa/Romeo, fator de impacto)
Publicador Amer Soc Microbiology
Extensão 2102-2114
Fonte http://dx.doi.org/10.1128/IAI.74.4.2102-2114.2006
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000236477000012
Endereço permanente http://repositorio.unifesp.br/handle/11600/28803

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