Praziquantel and albendazole damaging action on in vitro developing Mesocestoides corti (Platyhelminthes : Cestoda)

Praziquantel and albendazole damaging action on in vitro developing Mesocestoides corti (Platyhelminthes : Cestoda)

Autor Markoski, M. M. Google Scholar
Trindade, E. S. Google Scholar
Cabrera, G. Google Scholar
Laschuk, A. Google Scholar
Galanti, N. Google Scholar
Zaha, A. Google Scholar
Nader, H. B. Google Scholar
Ferreira, H. B. Google Scholar
Instituição Univ Fed Rio Grande Sul
Universidade Federal de São Paulo (UNIFESP)
Univ Chile
Resumo Parasitic flatworms present several steps of body architecture rearrangement during their fast transition from one developmental stage to another, which are, at least in part, responsible for their evasion from host immune response. Besides, different developmental stages present different degrees of susceptibility to drug action, and the identification of more susceptible stages is of importance for the definition of therapeutical approaches. Mesocastoides corti (syn. Mesocestoides vogae) is considered a good model to Study cestode biology because it can be easily manipulated both in vivo and in vitro and due to its relatively close relationship to cestodes of medical relevance, such as those from genera Echinococcus or Taenia. We have analyzed the damaging action of two broad spectrum anthelmintic drugs (praziquantel and albendazole) throughout the in vitro strobilization process of M. corti in order to identify developmental stages or body structures more Susceptible to these drugs. Tetrathyridia (larval stage) and segmented-induced worms were cultivated and treated with praziquantel and albendazole. Whole mounted samples, taken from different developmental stages, were fixed and stained with fluorophore-labeled WGA lectin and phalloidin for the analysis of tegument and Muscles, respectively. Conflocal laser scanning microscopy was used to identify anatomical changes and lesions caused by each anthelmintic drug in a 3D view. We demonstrated that both praziquantel and albendazole cause extensive tissue damage, especially on tegument, and that adult forms were the most Susceptible to drug exposure. (c) 2005 Elsevier Ireland Ltd. All rights reserved.
Palavra-chave Mesocestoides corti
anthelmintic drugs
confocal microscopy
in vitro development
Idioma Inglês
Data de publicação 2006-03-01
Publicado em Parasitology International. Clare: Elsevier B.V., v. 55, n. 1, p. 51-61, 2006.
ISSN 1383-5769 (Sherpa/Romeo, fator de impacto)
Publicador Elsevier B.V.
Extensão 51-61
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000235301100010
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