Granuloma formation in vitro requires B-1 cells and is modulated by Paracoccidioides brasiliensis gp43 antigen

Granuloma formation in vitro requires B-1 cells and is modulated by Paracoccidioides brasiliensis gp43 antigen

Author Vigna, A. F. Google Scholar
Almeida, SR Google Scholar
Xander, Patricia Autor UNIFESP Google Scholar
Freymuller, E. Google Scholar
Mariano, M. Google Scholar
Lopes, J. D. Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Abstract The mechanisms that determine granuloma formation and the significance of this type of inflammatory response in the pathogenesis of fungal diseases such as paracoccidioidomycosis are far from fully understood. We developed a granuloma model in vitro using beads to evaluate the role of isolated mouse peritoneal macrophages and B-1 cells. We also investigated granuloma formation in the presence of gp43, the main antigenic component of Paracoccidioides brasiliensis, which is secreted exocellularly. To determine whether B-1 cells, macrophages, or both, participate in granuloma formation, peritoneal cells from Xid mice, which lack B-1 cells, were used. Granuloma-like structures were not formed with Xid peritoneal cells or with cells from wild type mice that had their peritoneal and pleural cavities irradiated before the cultures were established. Granulomas were observed either when total adherent peritoneal cells or when isolated B-1 cells were added to macrophage cultures. the data strongly suggest that an interaction of B-1 cells and macrophages plays an important role in granuloma-like formation in this experimental model and that the presence of gp43 strongly stimulates this response. (c) 2005 Elsevier SAS. All rights reserved.
Keywords B-1 cells
in vitro granuloma
Paracoccidioides brasiliensis
Language English
Date 2006-03-01
Published in Microbes and Infection. Amsterdam: Elsevier B.V., v. 8, n. 3, p. 589-597, 2006.
ISSN 1286-4579 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 589-597
Access rights Closed access
Type Article
Web of Science ID WOS:000237754000001

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