Investigation of mitochondrial involvement in the experimental model of epilepsy induced by pilocarpine

Investigation of mitochondrial involvement in the experimental model of epilepsy induced by pilocarpine

Autor Nasseh, I. E. Google Scholar
Amado, D. Google Scholar
Cavalheiro, E. A. Google Scholar
Naffah-Mazzacoratti, M. D. Google Scholar
Tengan, C. H. Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Resumo Mitochondrial abnormalities have been associated with several aspects of epileptogenesis, such as energy generation, control of cell death, neurotransmitter synthesis, and free radical (FR) production. Increased production of FRs may cause mtDNA damage leading to decreased activities of oxidative phosphorylation complexes containing mtDNA-encoded subunits. in this study, we investigated whether increased generation of FR during status epilepticus would be sufficient to provoke abnormalities in mtDNA and in the expression and activity of cytochrome c oxidase (CCO), complex IV of the respiratory chain, in the chronic phase of the pilocarpine model of temporal lobe epilepsy. DNA analysis revealed low amounts of a 4.8 kb mtDNA deletion but with no differences in frequency or quantity in the control and experimental groups. We did not find abnormalities in the expression and distribution of an mtDNA-encoded subunit of CCO (CCO-I) or a relative decrease in CCO-I when compared with nuclear-encoded subunits (CCO-IV and SDH-fp). No abnormality in CCO activity was observed through histochemistry. Although evidences of mitochondrial abnormalities were found in previously published studies, our results do not suggest that the FRs, generated during the acute phase, determined important abnormalities in mtDNA, in expression of CCO-I, and in CCO activity. (c) 2005 Elsevier B.V. All tights reserved.
Palavra-chave epilepsy
pilocarpine
mitochondria
mitochondrial DNA
cytochrome c oxidase
free radical
Idioma Inglês
Data de publicação 2006-03-01
Publicado em Epilepsy Research. Amsterdam: Elsevier B.V., v. 68, n. 3, p. 229-239, 2006.
ISSN 0920-1211 (Sherpa/Romeo, fator de impacto)
Publicador Elsevier B.V.
Extensão 229-239
Fonte http://dx.doi.org/10.1016/j.eplepsyres.2005.11.009
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000236294500005
Endereço permanente http://repositorio.unifesp.br/handle/11600/28770

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